Subtilase enzymes

A modified subtilase comprising a mutation in an amino acid sequence of a subtilase, wherein the mutation is an insertion of at least one additional amino acid residue at position 126 of the active site loop (c) region corresponding to positions 125 to 132, wherein the positions are numbered according to the amino acid sequence of the mature subtilisin BPN′.

A modified subtilase comprising a mutation in an amino acid sequence of a subtilase, wherein the mutation is an insertion of at least one additional amino acid residue at position 127 of the active site loop (c) region corresponding to positions 125 to 132, wherein the positions are numbered according to the amino acid sequence of the mature subtilisin BPN′.

A modified subtilase comprising a mutation in an amino acid sequence of a subtilase, wherein the mutation is an insertion of at least one additional amino acid residue at position 128 of the active site loop (c) region corresponding to positions 125 to 132, wherein the positions are numbered according to the amino acid sequence of the mature subtilisin BPN′.

A modified subtilase comprising a mutation in an amino acid sequence of a subtilase, wherein the mutation is an insertion of at least one additional amino acid residue at position 129 of the active site loop (c) region corresponding to positions 125 to 132, wherein the positions are numbered according to the amino acid sequence of the mature subtilisin BPN′.

A modified subtilase comprising a mutation in an amino acid sequence of a subtilase, wherein the mutation is an insertion of at least one additional amino acid residue at position 130 of the active site loop (c) region corresponding to positions 125 to 132, wherein the positions are numbered according to the amino acid sequence of the mature subtilisin BPN′.

A modified subtilase comprising a mutation in an amino acid sequence of a subtilase, wherein the mutation is an insertion of at least one additional amino acid residue at position 131 of the active site loop (c) region corresponding to positions 125 to 132, wherein the positions are numbered according to the amino acid sequence of the mature subtilisin BPN′.

A modified subtilase comprising a mutation in an amino acid sequence of a subtilase, wherein the mutation is an insertion of at least one additional amino acid residue at position 132 of the active site loop (c) region corresponding to positions 125 to 132, wherein the positions are numbered according to the amino acid sequence of the mature subtilisin BPN′.

A subtilase comprising at least nine amino acid residues in the active site loop (c) region corresponding to positions 125 to 132, wherein the positions are numbered according to the amino acid sequence of the mature subtilisin BPN′.

A subtilase belonging to the I-S1 sub-group having the amino acid sequence (SEQ ID NO:

• 23):
• 9 1 10 20 30 A-Q-T-V-P-Y-G-I-P-L-I-K-A-D-K-V-Q-A-Q-G-F-K-G-A-N-V-K-V-A-V 40 50 60 L-D-T-G-I-Q-A-S-H-P-D-L-N-V-V-G-G-A-S-F-V-A-G-E-A-*-Y-N-T-D 70 80 90 G-N-G-H-G-T-H-V-A-G-T-V-A-A-L-D-N-T-T-G-V-L-G-V-A-P-S-V-S-L 100 110 120 Y-A-V-K-V-L-N-S-S-G-S-G-T-Y-S-G-I-V-S-G-I-E-W-A-T-T-N-G-M-D 125a 130 140 150 V-I-N-M-S-X-L-G-G-P-S-G-S-T-A-M-K-Q-A-V-D-N-A-Y-A-R-G-V-V-V-V 160 170 180 A-A-A-G-N-S-G-S-S-G-N-T-N-T-I-G-Y-P-A-K-Y-D-S-V-I-A-V-G-A-V 190 200 210 D-S-N-S-N-R-A-S-F-S-S-V-G-A-E-L-E-V-M-A-P-G-A-G-V-Y-S-T-Y-P 220 230 240 T-S-T-Y-A-T-L-N-G-T-S-M-A-S-P-H-V-A-G-A-A-A-L-I-L-S-K-H-P-N 250 260 270 L-S-A-S-Q-V-R-N-R-L-S-S-T-A-T-Y-L-G-S-S-F-Y-Y-G-K-G-L-I-N-V 275 E-A-A-A-Q
• or a homologous subtilase having an amino acid sequence comprising a position 125a amino acid residue and exhibiting an identity of more than 70%, 75%, 80%, 85%, 90%, or 95% therewith.

A subtilase belonging to the I-S2 sub-group having the amino acid sequence (SEQ ID NO:

• 24):
• 10 1 10 20 30 A-Q-S-V-P-W-G-I-S-R-V-Q-A-P-A-A-H-N-R-G-L-T-G-S-G-V-K-V-A-V- 40 50 60 L-D-T-G-I-*-S-T-H-P-D-L-N-I-R-G-G-A-S-F-V-P-G-E-P-*-S-T-Q-D- 70 80 90 G-N-G-H-G-T-H-V-A-G-T-I-A-A-L-N-N-S-I-G-V-L-G-V-A-P-S-A-E-L- 110 120 Y-A-V-K-V-L-G-A-S-G-S-G-S-V-S-S-I-A-Q-G-L-E-W-A-G-N-N-G-M-H- 125a 130 140 150 V-A-N-L-S-X-L-G-S-P-S-P-S-A-T-L-E-Q-A-V-N-S-A-T-S-R-G-V-L-V-V- 160 170 180 A-A-S-G-N-S-G-A-*-G-S-I-S-*-*-*-Y-P-A-R-Y-A-N-A-M-A-V-G-A-T- 190 200 210 D-Q-N-H-N-R-A-S-F-S-Q-Y-G-A-G-L-D-I-V-A-P-G-V-N-V-Q-S-T-Y-P- 220 230 240 G-S-T-Y-A-S-L-N-G-T-S-M-A-T-P-H-V-A-G-A-A-A-L-V-K-Q-K-N-P-S- 250 260 270 W-S-N-V-Q-I-R-N-H-L-K-N-T-A-T-S-L-G-S-T-N-L-Y-G-S-G-L-V-N-A- 275 E-A-A-T-R
• or a homologous subtilase having an amino acid sequence comprising a position 125a amino acid residue and exhibiting an identity of more than 70%, 75%, 80%, 85%, 90%, or 95% therewith.

A subtilase belonging to the I-S1 sub-group having the amino acid sequence (SEQ ID NO:

• 25):
• 11 1 10 20 30 A-Q-T-V-P-Y-G-I-P-L-I-K-A-D-K-V-Q-A-Q-G-F-K-G-A-N-V-K-V-A-V 40 50 60 L-D-T-G-I-Q-A-S-H-P-D-L-N-V-V-G-G-A-S-F-V-A-G-E-A-*-Y-N-T-D 70 80 90 G-N-G-H-G-T-H-V-A-G-T-V-A-A-L-D-N-T-T-G-V-L-G-V-A-P-S-V-S-L 100 110 120 Y-A-V-K-V-L-N-S-S-G-S-G-T-Y-S-G-I-V-S-G-I-E-W-A-T-T-N-G-M-D 126a 130 140 150 V-I-N-M-S-L-X-G-G-P-S-G-S-T-A-M-K-Q-A-V-D-N-A-Y-A-R-G-V-V-V-V 160 170 180 A-A-A-G-N-S-G-S-S-G-N-T-N-T-I-G-Y-P-A-K-Y-D-S-V-I-A-V-G-A-V 190 200 210 D-S-N-S-N-R-A-S-F-S-S-V-G-A-E-L-E-V-M-A-P-G-A-G-V-Y-S-T-Y-P 220 230 240 T-S-T-Y-A-T-L-N-G-T-S-M-A-S-P-H-V-A-G-A-A-A-L-I-L-S-K-H-P-N 250 260 270 L-S-A-S-Q-V-R-N-R-L-S-S-T-A-T-Y-L-G-S-S-F-Y-Y-G-K-G-L-I-N-V 275 E-A-A-A-Q
• or a homologous subtilase having an amino acid sequence comprising a position 126a amino acid residue and exhibiting an identity of more than 70%, 75%, 80%, 85%, 90%, or 95% therewith.

A subtilase belonging to the I-S2 sub-group having the amino acid sequence (SEQ ID NO:

• 26):
• 12 1 10 20 30 A-Q-S-V-P-W-G-I-S-R-V-Q-A-P-A-A-H-N-R-G-L-T-G-S-G-V-K-V-A-V- 40 50 60 L-D-T-G-I-*-S-T-H-P-D-L-N-I-R-G-G-A-S-F-V-P-G-E-P-*-S-T-Q-D- 70 80 90 G-N-G-H-G-T-H-V-A-G-T-I-A-A-L-N-N-S-I-G-V-L-G-V-A-P-S-A-E-L- 100 110 120 Y-A-V-K-V-L-G-A-S-G-S-G-S-V-S-S-I-A-Q-G-L-E-W-A-G-N-N-G-M-H- 126a 130 140 150 V-A-N-L-S-L-X-G-S-P-S-P-S-A-T-L-E-Q-A-V-N-S-A-T-S-R-G-V-L-V-V- 160 170 180 A-A-S-G-N-S-G-A-*-G-S-I-S-*-*-*-Y-P-A-R-Y-A-N-A-M-A-V-G-A-T- 190 200 210 D-Q-N-N-N-R-A-S-F-S-Q-Y-G-A-G-L-D-I-V-A-P-G-V-N-V-Q-S-T-Y-P- 220 230 240 G-S-T-Y-A-S-L-N-G-T-S-M-A-T-P-H-V-A-G-A-A-A-L-V-K-Q-K-N-P-S- 250 260 270 W-S-N-V-Q-I-R-N-H-L-K-N-T-A-T-S-L-G-S-T-N-L-Y-G-S-G-L-V-N-A- 275 E-A-A-T-R
• or a homologous subtilase having an amino acid sequence comprising a position 126a amino acid residue and exhibiting an identity of more than 70%, 75%, 80%, 85%, 90%, or 95% therewith.

A subtilase belonging to the I-S1 sub-group having the amino acid sequence (SEQ ID NO:

• 27):
• 13 1 10 20 30 A-Q-T-V-P-Y-G-I-P-L-I-K-A-D-K-V-Q-A-Q-G-F-K-G-A-N-V-K-V-A-V 40 50 60 L-D-T-G-I-Q-A-S-H-P-D-L-N-V-V-G-G-A-S-F-V-A-G-E-A-*-Y-N-T-D 70 80 90 G-N-G-H-G-T-H-V-A-G-T-V-A-A-L-D-N-T-T-G-V-L-G-V-A-P-S-V-S-L 100 110 120 Y-A-V-K-V-L-N-S-S-G-S-G-T-Y-S-G-I-V-S-G-I-E-W-A-T-T-N-G-M-D 127a 130 140 150 V-I-N-M-S-L-G-X-G-P-S-G-S-T-A-M-K-Q-A-V-D-N-A-Y-A-R-G-V-V-V-V 160 170 180 A-A-A-G-N-S-G-S-S-G-N-T-N-T-T-G-Y-P-A-K-Y-D-S-V-I-A-V-G-A-V 190 200 210 D-S-N-S-N-R-A-S-F-S-S-V-G-A-E-L-E-V-M-A-P-G-A-G-V-Y-S-T-Y-P 220 230 240 T-S-T-Y-A-T-L-N-G-T-S-M-A-S-P-H-V-A-G-A-A-A-L-I-L-S-K-H-P-N 250 260 270 L-S-A-S-Q-V-R-N-R-L-S-S-T-A-T-Y-L-G-S-S-F-Y-Y-G-K-G-L-I-N-V 275 E-A-A-A-Q
• or a homologous subtilase having an amino acid sequence comprising a position 127a amino acid residue and exhibiting an identity of more than 70%, 75%, 80%, 85%, 90%, or 95% therewith.

A subtilase belonging to the I-S2 sub-group having the amino acid sequence (SEQ ID NO:

• 28):
• 14 1 10 20 30 A-Q-S-V-P-W-G-I-S-R-V-Q-A-P-A-A-H-N-R-G-L-T-G-S-G-V-K-V-A-V- 40 50 60 L-D-T-G-I-*-S-T-H-P-D-L-N-I-R-G-G-A-S-F-V-P-G-E-P-*-S-T-Q-D- 70 80 90 G-N-G-H-G-T-H-V-A-G-T-I-A-A-L-N-N-S-I-G-V-L-G-V-A-P-S-A-E-L- 100 110 120 Y-A-V-K-V-L-G-A-S-G-S-G-S-V-S-S-I-A-Q-G-L-E-W-A-G-N-N-G-M-H- 127a 130 140 150 V-A-N-L-S-L-G-X-S-P-S-P-S-A-T-L-E-Q-A-V-N-S-A-T-S-R-G-V-L-V-V- 160 170 180 A-A-S-G-N-S-G-A-*-G-S-I-S-*-*-*-Y-P-A-R-Y-A-N-A-M-A-V-G-A-T- 190 200 210 D-Q-N-N-N-R-A-S-F-S-Q-Y-G-A-G-L-D-I-V-A-P-G-V-N-V-Q-S-T-Y-P- 220 230 240 G-S-T-Y-A-S-L-N-G-T-S-M-A-T-P-H-V-A-G-A-A-A-L-V-K-Q-K-N-P-S- 250 260 270 W-S-N-V-Q-I-R-N-H-L-K-N-T-A-T-S-L-G-S-T-N-L-Y-G-S-G-L-V-N-A- 275 E-A-A-T-R
• or a homologous subtilase having an amino acid sequence comprising a position 127a amino acid residue and exhibiting an identity of more than 70%, 75%, 80%, 85%, 90%, or 95% therewith.

A subtilase belonging to the I-S1 sub-group having the amino acid sequence (SEQ ID NO:

• 29):
• 15 1 10 20 30 A-Q-T-V-P-Y-G-I-P-L-I-K-A-D-K-V-Q-A-Q-G-F-K-G-A-N-V-K-V-A-V 40 50 60 L-D-T-G-I-Q-A-S-H-P-D-L-N-V-V-G-G-A-S-F-V-A-G-E-A-*-Y-N-T-D 70 80 90 G-N-G-H-G-T-H-V-A-G-T-V-A-A-L-D-N-T-T-G-V-L-G-V-A-P-S-V-S-L 100 110 120 Y-A-V-K-V-L-N-S-S-G-S-G-T-Y-S-G-I-V-S-G-I-E-W-A-T-T-N-G-M-D 128a 140 150 V-I-N-M-S-L-G-G-X-P-S-G-S-T-A-M-K-Q-A-V-D-N-A-Y-A-R-G-V-V-V-V 160 170 180 A-A-A-G-N-S-G-S-S-G-N-T-U-T-I-G-Y-P-A-K-Y-D-S-V-I-A-V-G-A-V 190 200 210 D-S-N-S-N-R-A-S-F-S-S-V-G-A-E-L-E-V-M-A-P-G-A-G-V-Y-S-T-Y-P 220 230 240 T-S-T-Y-A-T-L-N-G-T-S-M-A-S-P-H-V-A-G-A-A-A-L-I-L-S-K-H-P-N 250 260 270 L-S-A-S-Q-V-R-N-R-L-S-S-T-A-T-Y-L-G-S-S-F-Y-Y-G-K-G-L-I-N-V 275 E-A-A-A-Q
• or a homologous subtilase having an amino acid sequence comprising a position 128a amino acid residue and exhibiting an identity of more than 70%, 75%, 80%, 85%, 90%, or 95% therewith.

A subtilase belonging to the I-S2 sub-group having the amino acid sequence (SEQ ID NO:

• 30):
• 16 1 10 20 30 A-Q-S-V-P-W-G-I-S-R-V-Q-A-P-A-A-H-N-R-G-L-T-G-S-G-V-K-V-A-V- 40 50 60 L-D-T-G-I-*-S-T-H-P-D-L-N-I-R-G-G-A-S-F-V-P-G-E-P-*-S-T-Q-D- 70 80 90 G-N-G-H-G-T-H-V-A-G-T-I-A-A-L-N-N-S-I-G-V-L-G-V-A-P-S-A-E-L- 100 110 120 Y-A-V-K-V-L-G-A-S-G-S-G-S-V-S-S-I-A-Q-G-L-E-W-A-G-N-N-G-M-H- 128a 140 150 V-A-N-L-S-L-G-S-X-P-S-P-S-A-T-L-E-Q-A-V-N-S-A-T-S-R-G-V-L-V-V- 160 170 180 A-A-S-G-N-S-G-A-*-G-S-I-S-*-*-*-Y-P-A-R-Y-A-N-A-M-A-V-G-A-T- 190 200 210 D-Q-N-N-N-R-A-S-F-S-Q-Y-G-A-G-L-D-I-V-A-P-G-V-N-V-Q-S-T-Y-P- 220 230 240 G-S-T-Y-A-S-L-N-G-T-S-M-A-T-P-H-V-A-G-A-A-A-L-V-K-Q-K-N-P-S- 250 260 270 W-S-N-V-Q-I-R-N-H-L-K-N-T-A-T-S-L-G-S-T-N-L-Y-G-S-G-L-V-N-A- 275 E-A-A-T-R
• or a homologous subtilase having an amino acid sequence comprising a position 128a amino acid residue and exhibiting an identity of more than 70%, 75%, 80%, 85%, 90%, or 95% therewith.

A subtilase belonging to the I-S1 sub-group having the amino acid sequence (SEQ ID NO:

• 31):
• 17 1 10 20 30 A-Q-T-V-P-Y-G-I-P-L-I-K-A-D-K-V-Q-A-Q-G-F-K-G-A-N-V-K-V-A-V 40 50 60 L-D-T-G-I-Q-A-S-H-P-D-L-N-V-V-G-G-A-S-F-V-A-G-E-A-*-Y-N-T-D 70 80 90 G-N-G-H-G-T-H-V-A-G-T-V-A-A-L-D-N-T-T-G-V-L-G-V-A-P-S-V-S-L 100 110 120 Y-A-V-K-V-L-N-S-S-G-S-G-T-Y-S-G-I-V-S-G-I-E-W-A-T-T-N-G-M-D 129a 140 150 V-I-N-M-S-L-G-G-P-X-S-G-S-T-A-M-K-Q-A-V-D-N-A-Y-A-R-G-V-V-V-V 160 170 180 A-A-A-G-N-S-G-S-S-G-N-T-N-T-I-G-Y-P-A-K-Y-D-S-V-I-A-V-G-A-V 190 200 210 D-S-N-S-N-R-A-S-F-S-S-V-G-A-E-L-E-V-M-A-P-G-A-G-V-Y-S-T-Y-P 220 230 240 T-S-T-Y-A-T-L-N-G-T-S-M-A-S-P-H-V-A-G-A-A-A-L-I-L-S-K-H-P-N 250 260 270 L-S-A-S-Q-V-R-N-R-L-S-S-T-A-T-Y-L-G-S-S-F-Y-Y-G-K-G-L-I-N-V 275 E-A-A-A-Q
• or a homologous subtilase having an amino acid sequence comprising a position 129a amino acid residue and exhibiting an identity of more than 70%, 75%, 80%, 85%, 90%, or 95% there-with.

A subtilase belonging to the I-S2 sub-group having the amino acid sequence (SEQ ID NO:

• 32):
• 18 1 10 20 30 A-Q-S-V-P-W-G-I-S-R-V-Q-A-P-A-A-H-N-R-G-L-T-G-S-G-V-K-V-A-V- 40 50 60 L-D-T-G-I-*-S-T-H-P-D-L-N-I-R-G-G-A-S-F-V-P-G-E-P-*-S-T-Q-D- 70 80 90 G-N-G-H-G-T-H-V-A-G-T-I-A-A-L-N-N-S-I-G-V-L-G-V-A-P-S-A-E-L- 100 110 120 Y-A-V-K-V-L-G-A-S-G-S-G-S-V-S-S-I-A-Q-G-L-E-W-A-G-N-N-G-M-H- 129a 140 150 V-A-N-L-S-L-G-S-P-X-S-P-S-A-T-L-E-Q-A-V-N-S-A-T-S-R-G-V-L-V-V- 160 170 180 A-A-S-G-N-S-G-A-*-G-S-I-S-*-*-*-Y-P-A-R-Y-A-N-A-M-A-V-G-A-T- 190 200 210 D-Q-N-N-N-R-A-S-F-S-Q-Y-G-A-G-L-D-I-V-A-P-G-V-N-V-Q-S-T-Y-P- 220 230 240 G-S-T-Y-A-S-L-N-G-T-S-M-A-T-P-H-V-A-G-A-A-A-L-V-K-Q-K-B-P-S- 250 260 270 W-S-N-V-Q-I-R-N-H-L-K-N-T-A-T-S-L-G-S-T-N-L-Y-G-S-G-L-V-N-A- 275 E-A-A-T-R
• or a homologous subtilase having an amino acid sequence comprising a position 129a amino acid residue and exhibiting an identity of more than 70%, 75%, 80%, 85%, 90%, or 95% therewith.

A subtilase belonging to the I-S1 sub-group having the amino acid sequence (SEQ ID NO:

• 33):
• 19 1 10 20 30 A-Q-T-V-P-Y-G-I-P-L-I-K-A-D-K-V-Q-A-Q-G-F-K-G-A-N-V-K-V-A-V 40 50 60 L-D-T-G-I-Q-A-S-H-P-D-L-N-V-V-G-G-A-S-F-V-A-G-E-A-*-Y-N-T-D 70 80 90 G-N-G-H-G-T-H-V-A-G-T-V-A-A-L-D-N-T-T-G-V-L-G-V-A-P-S-V-S-L 100 110 120 Y-A-V-K-V-L-N-S-S-G-S-G-T-Y-S-G-I-V-S-G-I-E-W-A-T-T-N-G-M-D 130a 140 150 V-I-N-M-S-L-G-G-P-S-X-G-S-T-A-M-K-Q-A-V-D-N-A-Y-A-R-G-V-V-V-V 160 170 180 A-A-A-G-N-S-G-S-S-G-N-T-N-T-I-G-Y-P-A-K-Y-D-S-V-I-A-V-G-A-V 190 200 210 D-S-N-S-N-R-A-S-F-S-S-V-G-A-E-L-E-V-M-A-P-G-A-G-V-Y-S-T-Y-P 220 230 240 T-S-T-Y-A-T-L-N-G-T-S-M-A-S-P-H-V-A-G-A-A-A-L-I-L-S-K-H-P-N 250 260 270 L-S-A-S-Q-V-R-N-R-L-S-S-T-A-T-Y-L-G-S-S-F-Y-Y-G-K-G-L-I-N-V 275 E-A-A-A-Q
• or a homologous subtilase having an amino acid sequence comprising a position 130a amino acid residue and exhibiting an identity of more than 70%, 75%, 80%, 85%, 90%, or 95% therewith.

A subtilase belonging to the I-S2 sub-group having the amino acid sequence (SEQ ID NO:

• 34):
• 20 1 10 20 30 A-Q-S-V-P-W-G-I-S-R-V-Q-A-P-A-A-H-N-R-G-L-T-G-S-G-V-K-V-A-V- 40 50 60 L-D-T-G-I-*-S-T-H-P-D-L-N-I-R-G-G-A-S-F-V-P-G-E-P-*-S-T-Q-D- 70 80 90 G-N-G-H-G-T-H-V-A-G-T-I-A-A-L-N-N-S-I-G-V-L-G-V-A-P-S-A-E-L- 100 110 120 Y-A-V-K-V-L-G-A-S-G-S-G-S-V-S-S-I-A-Q-G-L-E-W-A-G-N-N-G-M-H- 130a 140 150 V-A-N-L-S-L-G-S-P-S-X-P-S-A-T-L-E-Q-A-V-N-S-A-T-S-R-G-V-L-V-V- 160 170 180 A-A-S-G-N-S-G-A-*-G-S-I-S-*-*-*-Y-P-A-R-Y-A-N-A-M-A-V-G-A-T- 190 200 210 D-Q-N-N-N-R-A-S-F-S-Q-Y-G-A-G-L-D-I-V-A-P-G-V-N-V-Q-S-T-Y-P- 220 230 240 G-S-T-Y-A-S-L-N-G-T-S-M-A-T-P-H-V-A-G-A-A-A-L-V-K-Q-K-N-P-S- 250 260 270 W-S-N-V-Q-I-R-N-H-L-K-N-T-A-T-S-L-G-S-T-N-L-Y-G-S-G-L-V-N-A- 275 E-A-A-T-R
• or a homologous subtilase having an amino acid sequence comprising a position 130a amino acid residue and exhibiting an identity of more than 70%, 75%, 80%, 85%, 90%, or 95% therewith.

A subtilase belonging to the I-S1 sub-group having the amino acid sequence (SEQ ID NO:

• 35):
• 21 1 10 20 30 A-Q-T-V-P-Y-G-I-P-L-I-K-A-D-K-V-Q-A-Q-G-F-K-G-A-N-V-K-V-A-V 40 50 60 L-D-T-G-I-Q-A-S-H-P-D-L-N-V-V-G-G-A-S-F-V-A-G-E-A-*-Y-N-T-D 70 80 90 G-N-G-H-G-T-H-V-A-G-T-V-A-A-L-D-N-T-T-G-V-L-G-V-A-P-S-V-S-L 100 110 120 Y-A-V-K-V-L-N-S-S-G-S-G-T-Y-S-G-I-V-S-G-I-E-W-A-T-T-N-G-M-D 131a 140 150 V-I-N-M-S-L-G-G-P-S-G-X-S-T-A-M-K-Q-A-V-D-N-A-Y-A-R-G-V-V-V-V 160 170 180 A-A-A-G-N-S-G-S-S-G-N-T-N-T-I-G-Y-P-A-K-Y-D-S-V-I-A-V-G-A-V 190 200 210 D-S-N-S-N-R-A-S-F-S-S-V-G-A-E-L-E-V-M-A-P-G-A-G-V-Y-S-T-Y-P 220 230 240 T-S-T-Y-A-T-L-N-G-T-S-M-A-S-P-H-V-A-G-A-A-A-L-I-L-S-K-H-P-N 250 260 270 L-S-A-S-Q-V-R-N-R-L-S-S-T-A-T-Y-L-G-S-S-F-Y-Y-G-K-G-L-I-N-V 275 E-A-A-A-Q
• or a homologous subtilase having an amino acid sequence comprising a position 131a amino acid residue and exhibiting an identity of more than 70%, 75%, 80%, 85%, 90%, or 95% there-with.

A subtilase belonging to the I-S2 sub-group having the amino acid sequence (SEQ ID NO:

• 36):
• 22 1 10 20 30 A-Q-S-V-P-W-G-I-S-R-V-Q-A-P-A-A-H-N-R-G-L-T-G-S-G-V-K-V-A-V- 40 50 60 L-D-T-G-I-*-S-T-H-P-D-L-N-I-R-G-G-A-S-F-V-P-G-E-P-*-S-T-Q-D- 70 80 90 G-N-G-H-G-T-H-V-A-G-T-I-A-A-L-N-N-S-I-G-V-L-G-V-A-P-S-A-E-L- 100 110 120 Y-A-V-K-V-L-G-A-S-G-S-G-S-V-S-S-I-A-Q-G-L-E-W-A-G-N-N-G-M-H- 131a 140 150 V-A-N-L-S-L-G-S-P-S-P-X-S-A-T-L-E-Q-A-V-N-S-A-T-S-R-G-V-L-V-V- 160 170 180 A-A-S-G-N-S-G-A-*-G-S-I-S-*-*-*-Y-P-A-R-Y-A-N-A-M-A-V-G-A-T- 190 200 210 D-Q-N-N-N-R-A-S-F-S-Q-Y-G-A-G-L-D-I-V-A-P-G-V-N-V-Q-S-T-Y-P- 220 230 240 G-S-T-Y-A-S-L-N-G-T-S-M-A-T-P-H-V-A-G-A-A-A-L-V-K-Q-K-N-P-S- 250 260 270 W-S-N-V-Q-I-R-N-H-L-K-N-T-A-T-S-L-G-S-T-N-L-Y-G-S-G-L-V-N-A- 275 E-A-A-T-R
• or a homologous subtilase having an amino acid sequence comprising a position 131a amino acid residue and exhibiting an identity of more than 70%, 75%, 80%, 85%, 90%, or 95% therewith.

A subtilase belonging to the I-S1 sub-group having the amino acid sequence (SEQ ID NO:

• 37):
• 23 1 10 20 30 A-Q-T-V-P-Y-G-I-P-L-I-K-A-D-K-V-Q-A-Q-G-F-K-G-A-N-V-K-V-A-V 40 50 60 L-D-T-G-I-Q-A-S-H-P-D-L-N-V-V-G-G-A-S-F-V-A-G-E-A-*-Y-N-T-D 70 80 90 G-N-G-H-G-T-H-V-A-G-T-V-A-A-L-D-N-T-T-G-V-L-G-V-A-P-S-V-S-L 100 110 120 Y-A-V-K-V-L-N-S-S-G-S-G-T-Y-S-G-I-V-S-G-I-E-W-A-T-T-N-G-M-D 132a 140 150 V-I-N-M-S-L-G-G-P-S-G-S-X-T-A-M-K-Q-A-V-D-N-A-Y-A-R-G-V-V-V-V 160 170 180 A-A-A-G-N-S-G-S-S-G-N-T-N-T-I-G-Y-P-A-K-Y-D-S-V-I-A-V-G-A-V 190 200 210 D-S-N-S-N-R-A-S-F-S-S-V-G-A-E-L-E-V-M-A-P-G-A-G-V-Y-S-T-Y-P 220 230 240 T-S-T-Y-A-T-L-N-G-T-S-M-A-S-P-H-V-A-G-A-A-A-L-I-L-S-K-H-P-N 250 260 270 L-S-A-S-Q-V-R-N-R-L-S-S-T-A-T-Y-L-G-S-S-F-Y-Y-G-K-G-L-I-N-V 275 E-A-A-A-Q
• or a homologous subtilase having an amino acid sequence comprising a position 132a acid residue and exhibiting an identity of more than 70%, 75%, 80%, 85%, 90%, or 95% there-with.

A subtilase belonging to the I-S2 sub-group having the amino acid sequence (SEQ ID NO:

• 38):
• 24 1 10 20 30 A-Q-S-V-P-W-G-I-S-R-V-Q-A-P-A-A-H-N-R-G-L-T-G-S-G-V-K-V-A-V- 40 50 60 L-D-T-G-I-*-S-T-H-P-D-L-N-I-R-G-G-A-S-F-V-P-G-E-P-*-S-T-Q-D- 70 80 90 G-N-G-H-G-T-H-V-A-G-T-I-A-A-L-N-N-S-I-G-V-L-G-V-A-P-S-A-E-L- 100 110 120 Y-A-V-K-V-L-G-A-S-G-S-G-S-V-S-S-I-A-Q-G-L-E-W-A-G-N-N-G-M-H- 132a 140 150 V-A-N-L-S-L-G-S-P-S-P-S-X-A-T-L-E-Q-A-V-N-S-A-T-S-R-G-V-L-V-V- 160 170 180 A-A-S-G-N-S-G-A-*-G-S-I-S-*-*-*-Y-P-A-R-Y-A-N-A-M-A-V-G-A-T- 190 200 210 D-Q-N-N-N-R-A-S-F-S-Q-Y-G-A-G-L-D-I-V-A-P-G-V-N-V-Q-S-T-Y-P- 220 230 240 G-S-T-Y-A-S-L-N-G-T-S-M-A-T-P-H-V-A-G-A-A-A-L-V-K-Q-K-N-P-S- 250 260 270 W-S-N-V-Q-I-R-N-H-L-K-N-T-A-T-S-L-G-S-T-N-L-Y-G-S-G-L-V-N-A- 275 E-A-A-T-R
• or a homologous subtilase having an amino acid sequence comprising a position 132a amino acid residue and exhibiting an identity of more than 70%, 75%, 80%, 85%, 90%, or 95% therewith.

A method for producing a subtilase or a subtilase variant of any of claims 193-237, wherein a host of any of claims 265-267 is cultured under conditions conducive to the expression and secretion of said variant, and the variant is recovered.