Method to control gene expression in bacteria, namely Rhizobiaceae, to improve root nodule development, nitrogen fixation and plant biomass production
OwnerRoberto Defez, Angelo Spenainventors
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Abstract
A promintron sequence derived from an intervening sequence of the rolA gene of Agrobacterium rhizogenes strain A4 is described. The sequence is able to drive gene expression within bacteroids in all stages of nodule development in order to obtain, over the developmental time of the nodule, a constitutive expression of the gene(s) of interest. Uses of said sequence, derived vectors and recombinant bacteria are also described.
Claims21
Use of the promintron sequence of the rolA gene from Agrobacterium rhizogenes as in SEQ ID NO. 1, or of DNA sequences comprising said promintron sequence, or of functional homologous or portion thereof, to induce the expression of a DNA coding sequence, in recombinant bacteria during exponential, post-exponential and stationary phase of growth, and in bacteroids within root nodules, said coding DNA sequence being under the control of said promintron sequence.
Genetically engineered bacteria comprising the recombinant DNA molecule according to claims from 6 to 13.
Use of the recombinant DNA molecule according to claims from 6 to 13 to significantly increase the size of nodules of a plant.
Use of the recombinant DNA molecule according to claims from 6 to 13 to significantly increase the capacity to fix nitrogen of a nodulated plant.
Use of the recombinant DNA molecule according to claims from 6 to 13 to significantly increase the plant biomass production.
Legume plant infected by bacteria harbouring the recombinant DNA molecule according to claims from 6 to 13 and having a significant increase of the size of nodules, and/or of the nodule capacity to fix nitrogen, and/or of the plant biomass, and/or of the ability to fix nitrogen.
Dependencies are inferred from the claim text.