Minicell-producing parent cells
OwnerMark W. Surber, Roger A. Sabbadini +2inventors
InventorsMark W. Surber, Roger A. Sabbadini, Anca M. Segall, Neil Berkley
Life of this patent
Estimating the term…
Abstract
The invention provides compositions and methods for the production of achromosomal and anucleate cells useful for applications such as diagnositic and therapeutic uses, as well as research tools and agents for drug discovery.
Claims20
A minicell-producing parent cell, wherein said parent cell comprises one or more of the following:
- (a) an expression element that comprises a gene operably linked to expression sequences that are inducible and/or repressible, wherein induction or repression of said gene regulates the copy number of an episomal expression construct;
- (b) a mutation in an endogenous gene, wherein said mutation regulates the copy number of an episomal expression construct;
- (c) an expression element that comprises a gene operably linked to expression sequences that are inducible and/or repressible, wherein induction or repression of said gene causes or enhances the production of minicells;
- and
- (d) a mutation in an endogenous gene, wherein said mutation causes or enhances minicell production.
A minicell-producing parent cell, wherein said parent cell comprises an expression construct, wherein said expression construct comprises expression sequences operably linked to an ORF that encodes a protein, and a regulatory expression element, wherein said regulatory expression element comprises expression sequences operably linked to a regulatory gene that encodes a factor that regulates the expression of said ORF.
Dependencies are inferred from the claim text.