Serum free cultivation of primate embryonic stem cells
OwnerJames A. Thomsoninventor
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Abstract
Disclosed herein are methods for culturing primate embryonic stem cells. These cells are cultured on a prolonged and stable basis in the presence of exogenously supplied fibroblast growth factor and in the absence of animal serum. Preferably there is also a fibroblast feeder layer. Also disclosed is a culture media containing fibroblast feeder layer and the fibroblast growth factor.
Claims16
A method of culturing primate embryonic stem cells, comprising:
- culturing the stem cells in a culture essentially free of mammalian fetal serum and in the presence of fibroblast growth factor that is supplied from a source other than just a fibroblast feeder layer.
4 The method of claim 2, wherein the fibroblast growth factor is basic fibroblast growth factor.
The method of claim 4, wherein the fibroblast growth factor is human basic fibroblast growth factor which has been produced from a recombinant gene.
A method of culturing primate embryonic stem cells, comprising:
- culturing the stem cells in a culture essentially free of mammalian fetal serum and in the presence of a growth factor capable of activating a fibroblast growth factor signaling receptor, wherein the growth factor is supplied from a source other than just a fibroblast feeder layer.
A culture system for culturing primate embryonic stem cells, comprising:
- a fibroblast feeder layer;
- and
- fibroblast growth factor supplied by other than just the fibroblast layer;
- wherein the culture system is essentially free of animal serum.
Dependencies are inferred from the claim text.