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AssigneeAbbott Laboratories
InventorsPradip Mukerji, Yung-Sheng Huang, Tapas Das, Jennifer Thurmond, Suzette L. Pereira
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The subject invention relates to the identification of genes involved in the desaturation of polyunsaturated fatty acids at carbon 5 (i.e., “Δ5-desaturase”) and at carbon 6 (i.e., “Δ6-desaturase”) and to uses thereof. In particular, Δ5-desaturase may be utilized, for example, in the conversion of dihomo-γ -linolenic acid (DGLA) to arachidonic acid (AA) and in the conversion of 20:4n-3 to eicosapentaenoic acid (EPA). Delta-6 desaturase may be used, for example, in the conversion of linoleic (LA) to γ-linolenic acid (GLA). AA or polyunsaturated fatty acids produced therefrom may be added to pharmaceutical compositions, nutritional compositions, animal feeds, as well as other products such as cosmetics.
An isolated nucleotide sequence corresponding to or complementary to at least about 50% of the nucleotide sequence selected from the group consisting of SEQ ID NO:13, SEQ ID NO:19 and SEQ ID NO:28.
A purified polypeptide which desaturates polyunsaturated fatty acids at carbon 5 and has at least about 50% amino acid similarity to the amino acid sequence comprising SEQ ID NO:20 or SEQ ID NO:29.
A purified polypeptide which desaturates polyunsaturated fatty acids at carbon 6 and has at least about 50% amino acid similarity to the amino acid sequence comprising SEQ ID NO:14.
A method of producing a desaturase comprising the steps of:
A vector comprising:
A transgenic, non-human mammal whose genome comprises a DNA sequence encoding an enzyme selected from the group consisting of Δ5-desaturase and Δ6-desaturase, operably linked to a promoter.
A method for producing a polyunsaturated fatty acid comprising the steps of:
A method for producing a polyunsaturated fatty acid comprising the steps of:
Dependencies are inferred from the claim text.