Fermentation process for the preparation of L-threonine
AssigneeDegussa AG
InventorsThomas Hermann, Mechthild Rieping
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Abstract
The invention relates to a process for the fermentative preparation of L-threonine in which an L-threonine-producing microorganism of the Enterobacteriaceae family is cultured by the feed process, and a portion of the fermentation broth is then separated off in order to be utilized for inoculation of further media.
Claims12
Process for the fermentative preparation of L-threonine, wherein
- a) an L-threonine-producing microorganism of the Enterobacteriaceae family is cultured by the feed process (fed batch), subsequently
- b) a portion of the fermentation broth is separated off, 1 to 90 vol. %, in particular 1 to 50 vol. %, preferably 1 to 25 vol. % of the total volume of the fermentation broth remaining in the fermentation tank, subsequently
- c) the remaining fermentation broth is topped up with growth medium and, preferably after a growth phase, a further fermentation is carried out in accordance with a),
- d) steps b) and c) are optionally carried out several times, and
- e) the L-threonine is isolated from the fermentation broths collected.
L-Threonine-producing and -secreting microorganisms of the Enterobacteriaceae family which have an enhanced resistant aspartate kinase I-homoserine dehydrogenase I, an attenuated threonine deaminase, a resistance to at least 5 g/l threonine and the ability to utilize sucrose as a source of carbon, and the parB gene region.
Dependencies are inferred from the claim text.