US 2003/0190690 A1 · Biosensor and method for analyzing blood components using it

Abstract

An object of the present invention is to provide a biosensor which enables a measurement with a small amount of specimen, for which whole blood with a reduced influence of blood cell can be used without previously separating blood plasma components from the blood, in an analysis of a component in the blood. The biosensor comprises a sample introductory part (11) to which a sample solution is applied, a space forming material (9) for forming the sample introductory part, a part (10) where a cell contraction agent for causing cellular components in the sample solution to constrict is held, a sample holding part (6) where the sample solution is held, a developing layer (2) for developing the sample solution, a marker reagent holding part (3) where a marker reagent is held, and a reagent immobilization part (5) as an area on the developing layer (2) where a specific protein is held. Further, a retiform structure (7) is provided between the space forming material (9) and the developing layer (2).

Drawings

Claims37

Dependent claims

A biosensor made of a dried porous material,

said biosensor comprising:
a sample introductory part for introducing a sample solution;
and a developing layer for developing the sample solution, wherein
the developing layer includes:
a marker reagent holding part where a marker reagent is held in a dry state so that it can be eluted by the development of the sample solution;
and a reagent immobilization part where a reagent which can bind to an analyte and is involved in a reaction is immobilized so that it is not eluted, and when the sample solution is introduced to the sample introductory part, the sample solution permeates the developing layer to reach the marker reagent holding part, and moves to the reagent immobilization part while eluting the marker reagent, whereby the reaction among the analyte, the marker reagent, and the immobilized reagent occurs, and
an amount of marker reagent bound in the reagent immobilization part is measured, thereby qualitatively or quantitatively measuring the analyte included in the sample solution, wherein
a retiform structure is arranged in the sample introductory part.

5 more elements

A method for analyzing a constituent of blood by employing a biosensor made of a dried porous material,

said biosensor comprising:
a developing layer for developing a sample solution;
a space forming part for forming a space on the developing layer;
and a sample introductory part having a cavity in which the sample solution flows, in the formed space, wherein
the developing layer includes:
a marker reagent holding part where a marker reagent is held in a dry state so that it can be eluted by the development of the sample solution;
and a reagent immobilization part where a reagent which can bind to an analyte and is involved in a reaction is immobilized so that it is not eluted, and when the sample solution is introduced to the sample introductory part, the sample solution permeates the developing layer to reach the marker reagent holding part, and moves to the reagent immobilization part while eluting the marker reagent, whereby the reaction among the analyte, the marker reagent, and the immobilized reagent occurs, and
said analysis method comprising:
measuring an amount of marker reagent bound in the reagent immobilization part, thereby qualitatively or quantitatively measuring the analyte included in the sample solution, wherein
a retiform structure is arranged in the sample introductory part, and the blood is developed.

7 more elements

A method for analyzing a constituent of blood by employing a biosensor made of a dried porous material,

said biosensor comprising:
a developing layer for developing a sample solution;
a space forming part for forming a space on the developing layer;
and a sample introductory part having a cavity in which the sample solution flows, in the formed space, wherein
a reagent holding part where a marker reagent is held in a dry state so that it can be eluted by the flow of the sample solution is provided in the cavity, and the developing layer includes a reagent immobilization part where a reagent which can bind to an analyte and is involved in a reaction is immobilized so that it is not eluted, and when the sample solution is introduced to the sample introductory part, the sample solution is developed on the developing layer while eluting the marker reagent, and reaches the reagent immobilization part, whereby the reaction among the analyte, the marker reagent, and the immobilized reagent occurs, and
said analysis method comprising:
measuring an amount of the marker reagent bound in the reagent immobilization part, thereby qualitatively or quantitatively measuring the analyte included in the sample solution, wherein
a retiform structure is arranged in the sample introductory part, and the blood is developed.

5 more elements

Dependencies are inferred from the claim text.

Description10,269 words

Summary6,613 words

#The present invention relates to a biosensor and, more particularly, to a dry-type biosensor for analyzing an analyte in a sample solution, and a method for analyzing a constituent of blood by employing the biosensor.

#As a means for diagnosing health conditions of persons, a biochemical examination of bodily fluids, especially blood, is widely conducted. A measuring method by a chromatography sensor, which utilizes an antigen-antibody reaction is generally used as a method for analyzing the bodily fluids. However, it is difficult to determine a kind or measure the concentration of blood constituent such as a metabolic product, a protein, a fat, an electrolyte, an enzyme, an antigen, and an antibody, by employing whole blood as it is. Thus, conventionally a general blood-constituent analysis method requires several operation processes, such as centrifuging blood which is previously collected, and analyzing the blood constituent employing obtained blood plasma or blood serum by an analytical instrument or a biosensor. However, when this method is employed, the measurement requires a special apparatus, and the preprocessing as well as the inspection take a lot of time. Thus, the centrifugation method which requires a centrifugal machine is not adequate when a small number of specimens are to be processed quickly or when inspections in the field is performed. Further, the obtained blood serum or blood plasma is smaller in amount as compared with the amount of blood.

#In recent years, a device which enables a quick, simple, and accurate measurement is desired under a concept of POC (Point of Care) in the medical diagnosis scene. However, in the conventional method as described above, in the case where a sample solution is to be applied to a sensor part, for example, when the sample solution is blood, a series of operations as described below is required. That is, blood is collected by the use of an injector, blood cells as material components and blood plasma are separated by the use of a centrifugal machine or the like in general, and the separated blood is applied to the sensor part with the use of an instrument such as a dispenser and a dropper. In this method, special skills in medical technology or the like are required to collect blood employing an injector, and further special apparatus and skills are required for the operation of centrifugal separation, and therefore this method cannot be employed in general households or when individuals without such techniques perform measurements for themselves. Further, since the instrument such as a dispenser is required to quantitatively measure the sample solution, the operation becomes complicated.

Brief description of the drawings8 figures

#FIG. 1 is a diagram of a biosensor according to a first embodiment of the present invention.

#FIG. 2 is a diagram of a biosensor according to a second embodiment of the present invention.

#FIG. 3 is a diagram of the biosensor according to the second embodiment of the invention.

Detailed description3,511 words

#Hereinafter, embodiments according to the present invention will be described with reference to the drawings. The embodiments described here are given only as examples and the present invention is not restricted to these embodiments.

#(Embodiment 1)

#A first embodiment of the present invention will be described with reference to the drawing.

Biosensor and method for analyzing blood components using it

Life of this patent

Biosensor and method for analyzing blood components using it

Life of this patent

Life of this patent

Abstract

Drawings

Drawings

Claims37

Description10,269 words

Drawings

Classification2 codes

Life of this patent

Description10,269 words