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US 2003/0190684 A1Published application
Multiparameter facs assays to detect alterations in cellular parameters and to screen small molecule libraries
AssigneeRigel, Inc.
InventorsJOSEPH FISHER, JAMES LORENS, DONALD PAYAN, ALEXANDER ROSSI
Life of this patent
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Abstract
The invention relates to novel methods of detecting alterations in cell cycle regulation in a cell or a cell population and screening for agents capable of modulating cell cycle regulation through the use of multiparameter assays and a fluorescence-activated cell sorter (FACS) machine.
Claims10
A method of screening for a bioactive agent capable of altering a cellular phenotype, said method comprising:
- a) combining at least one candidate bioactive agent and a population of cells;
- and
- b) sorting said cells in a FACS machine by separating said cells on the basis of at least five cellular parameters.
A method of screening for a bioactive agent capable of altering a cellular phenotype, said method comprising:
- a) introducing a library of nucleic acids each encoding a candidate bioactive agent into a population of cells;
- and
- b) sorting said cells in a FACS machine by separating said cells on the basis of at least three cellular parameters.
Dependencies are inferred from the claim text.