Consensus phytases
AssigneeRoche Vitamins Inc.
InventorMartin Lehmann
Life of this patent
Estimating the term…
Abstract
A process for obtaining a consensus protein from a group of amino acid sequences of a defined protein family, proteins and polynucleotides so obtained, and compositions containing such proteins.
Claims23
A process for obtaining a consensus protein from a group of amino acid sequences of a defined protein family, which comprises:
- a) aligning a group consisting of three to one hundred amino acid sequences from a defined protein family;
- b) comparing the evolutionary similarity of amino acids which occupy a position in the aligned sequences to select a consensus amino acid for said position using a system which is so organized that if two amino acids which occupy said position are identical, then the identical amino acid is selected as the consensus amino acid for said position, unless three or more other amino acids at said position have a higher degree of structural similarity to each other than to the identical amino acid, in which case the amino acid which has the highest degree of evolutionary similarity to the other amino acids is selected as the consensus amino acid for said position, with the proviso that if a set of amino acid sequences exists within the group of step a) such that the amino acid sequences within the set have more evolutionary simularity to each other than to any of the amino acid sequences of the group which are not part of the set, then the amino acids which occupy said position in members of the set will have a vote weight of one divided by the number of amino acid sequences in the set where the amino acids which occupy said position in amino acid sequences which are not in the set will have a vote weight of one, and repeating the procedure for each position in the aligned group of amino acid sequences;
- c) if no consensus amino acid for said position is obtained by the method of step b), then any amino acid at said position is selected as the consensus sequence;
- d) combining the consensus amino acids obtained in steps b) and c) to obtain a consensus amino acid sequence;
- e) translating the consensus amino acid sequence into a DNA sequence;
- f) obtaining the DNA sequence and using said DNA sequence to express a protein which is the consensus protein of the defined protein family.
A consensus protein which has the amino acid sequence shown in FIG. 2 or a variant or mutein thereof.
A consensus protein which has the amino acid sequence shown in FIG. 1 as consensus phytase or a variant or mutein thereof.
A mutein which has the amino acid sequence of FIG. 2 except that Q at position 50 has been replaced by L, T, or G.
A mutein which has the amino acid sequence of FIG. 2 except that Q at position 50 has been replaced by T and Y at position 51 has been replaced by N.
A mutein which has the amino acid sequence of FIG. 2 except that Q at position 50 has been replaced by L and Y at position 51 has been replaced by N.
Dependencies are inferred from the claim text.