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AssigneeMcGill University
InventorsRoger K. Prichard, Ming Xu, Ana Paula Ribeiro, William J. Blackhall, Robin N. Beech, Marcelo Molento, Hao Yuan Liu
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This invention describes novel purified and isolated nucleic acid molecules or the fragments thereof, extracted from nematode or arthropod pests or recombinant, which encode P-glycoprotein homologs and regulate resistance to the macrocyclic lactone compounds. The invention further relates to the new P-glycoprotein homolog expression product of these nucleic acids. Also described herein are methods for detecting the gene encoding for resistance to the macrocyclic lactone compounds in nematode or arthropod pests which comprise comparing the nucleic acids extracted from a pest specimen to the nucleic acids encoding for resistance and the nucleic acids encoding for susceptibility to the macrocyclic lactone compounds. Lastly, the present invention is also drawn to methods and compositions for increasing the efficacy of the macrocyclic lactone compounds against resistant nematode or resistant arthropod pests which comprise administering to a mammal or applying to crops and the like a pesticidal enhancing effective amount of a multidrug resistance reversing agent.
A purified and isolated nucleic acid molecule or a fragment thereof, extracted from a nematode or an arthropod pest, encoding a P-glycoprotein homolog which regulates resistance to a macrocyclic lactone compound.
A recombinant nucleic acid molecule or a fragment thereof encoding a P-glycoprotein homolog which regulates resistance to a macrocyclic lactone compound.
A method for detecting the resistance to a macrocyclic lactone compound in a nematode or an arthropod pest which comprises comparing a nucleic acid molecule extracted from a pest specimen to a nucleic acid molecule encoding for resistance to the macrocyclic lactone compound and a nucleic acid molecule encoding for susceptibility to the macrocyclic lactone compound.
A method for detecting the resistance to a macrocyclic lactone compound in a nematode or an arthropod pest which comprises preparing an antibody to a sequence of a peptide corresponding to the amino acid translation of a nucleic acid molecule or a fragment thereof encoding a P-glycoprotein homolog which regulates resistance to a macrocyclic lactone compound;
A method for increasing the efficacy of a macrocyclic lactone compound against a resistant crop pest which comprises applying to the crop, to the crop seed or to the soil or water in which the crop or the seed is growing or is to be grown a pesticidal enhancing effective amount of a multidrug resistance reversing agent.
A method for increasing the efficacy of a macrocyclic lactone compound against a resistant nematode or a resistant arthropod ectoparasite or endoparasite of a mammal which comprises administering a pesticidal enhancing effective amount of a multidrug resistance reversing agent to the mammal in connection with the administration of the macrocyclic lactone compound.
An improved composition for controlling or combatting a crop pest which comprises a pesticidally effective amount of a multidrug resistance reversing agent;
An improved composition for controlling or treating helminth or arthropod endo- or ectoparasitic insect infestation or infection of a mammal which comprises an anthelmintically or an arthropod endo- or ectoparasiticidally effective amount of a multidrug resistance reversing agent;
Dependencies are inferred from the claim text.