Methods of selecting T cell receptor V peptides for therapeutic use
AssigneesOregon Health & Science University, The Government of the United States of America, dba The Department of Veterans Affairs
InventorArthur A. Vandenbark
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Abstract
A method is disclosed to identify a T cell receptor (TCR) variable (V) peptide of use as a therapeutic agent in a subject. A method is also disclosed for monitoring the efficacy of a T Cell Receptor (TCR) V peptide for the treatment of a subject. In another embodiment, a method is disclosed for selecting a TCR V peptide of use in therapy for a subject having an autoimmune disease.
Claims37
A method of identifying a T cell receptor (TCR) variable (V) peptide of use as a therapeutic agent in a subject affected with a disorder, comprising:
- a) screening TCR V beta peptides, TCR V alpha peptides, or both TCR V beta peptides and TCR V alpha peptides to select a TCR V peptide that produces altered expression of a cytokine elicited in response to the TCR V peptide by T cells from the subject as compared to a control subject;
- b) determining a regulatory activity of CD4+CD25+ T cells isolated from the subject elicited in response to the TCR V polypeptide;
- thereby identifying a TCR V peptide of use as a therapeutic agent.
A method of monitoring the efficacy of a T Cell Receptor (TCR) V peptide for the treatment of a subject, comprising:
- exposing CD4+ cells from the subject to the TCR V peptide;
- and
- determining a T cell regulatory activity of CD4+CD25+ T cells isolated from the subject,
- wherein induction of regulatory activity indicates the efficacy of the TCR V peptide for the treatment of the subject.
A method of selecting a therapy for a subject having an autoimmune disease, comprising:
- a) identifying a T Cell Receptor Variable (TCR V) gene expressed by target T cells in the subject by screening for expression of a TCR V genes by activated T cells from the subject;
- and determining expression of a cytokine elicited in response to one or more TCR V peptides corresponding to the TCR V gene by T cells from the subject, thereby identifying a TCR V gene expressed by target T cells;
- and
- b) identifying a TCR V peptides corresponding to the TCR V gene that elicit T cell regulatory activity by a T cell isolated from the subject;
- thereby selecting a therapy that targets T cells expressing the TCR V gene.
Dependencies are inferred from the claim text.