Nucleic acid marker ladder for estimating mass
AssigneeInvitrogen Corporation
InventorJames L. Hartley
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Abstract
The invention relates to a nucleic acid marker ladder which is a restriction endonuclease digest, wherein a nucleic acid restriction endonuclease digest is a collection of nucleic acid fragments resulting from complete digestion of one or more nucleic acids by one or more restriction endonucleases; the restriction endonuclease digest contains at least 3 fragments; and the size of the fragments in base pairs is a multiple of an integer, wherein the integer is 10 or more.
Claims14
A nucleic acid marker ladder consisting essentially of a restriction endonuclease digest, wherein
- (a) the nucleic acid restriction endonuclease digest is a collection of nucleic acid fragments resulting from the complete digestion of one or more nucleic acids by one or more restriction endonucleases;
- (b) the restriction endonuclease digest contains at least 3 fragments;
- and
- (c) the size of the fragments in base pairs is a multiple of an integer, wherein the integer is 10 or more.
A nucleic acid marker kit comprising a carrier means having in close confinement therein at least one container means where the first container means contains a nucleic acid marker ladder consisting essentially of a restriction endonuclease digest, wherein
- (a) the nucleic acid restriction endonuclease digest is a collection of nucleic acid fragments resulting from the complete digestion of one or more nucleic acids by one or more restriction endonucleases;
- (b) the restriction endonuclease digest contains at least 3 fragments;
- and
- (c) the size of the fragments in base pairs is a multiple of an integer, wherein the integer is 10 or more.
A method of preparing a nucleic acid marker ladder comprising:
- (a) generating at least two polymerase chain reaction (PCR) products wherein each product is generated from a template comprising a restriction endonuclease site and a primer comprising the restriction endonuclease site in the template;
- (b) joining the PCR products to produce a nucleic acid molecule;
- and
- (c) completely digesting one or more nucleic acid molecules with at least one restriction endonuclease
- wherein a nucleic acid marker ladder is produced wherein the ladder contains at least 3 fragments and the size of the fragments in base pairs is a multiple of an integer, wherein the integer is 10 or more.
Dependencies are inferred from the claim text.