Compositions and methods for labeling of nucleic acid molecules
AssigneeInvitrogen Corporation
InventorsChristian E. Gruber, Po-Jen Shih
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Abstract
The present invention is generally related to compositions, kits and methods for labeling nucleic acid molecules using reverse transcriptases, preferably multi-subunit reverse transcriptases such as ASLV reverse transcriptases. Specifically, the invention relates to methods, kits and compositions for fluorescently labeling nucleic acid molecules during nucleic acid synthesis. The labeled nucleic acid molecules produced in accordance with the invention are particularly suited as labeled probes for nucleic acid detection and diagnostics.
Claims23
A composition for use in labeling one or more nucleic acid molecules, said composition comprising one or more enzymes having reverse transcriptase activity, wherein said enzymes are multi-subunit enzymes.
A method for reverse transcription of one or more nucleic acid molecules comprising
- (a) mixing one or more nucleic acid templates with one or more enzymes having reverse transcriptase activity, wherein said enzymes are multi-subunit enzymes;
- and
- (b) incubating said mixture under conditions sufficient to make one or more first nucleic acid molecules complementary to all or a portion of said one or more templates, wherein at least one of said first nucleic acid molecules is detectably labeled.
A kit for use in labeling one or more nucleic acid molecules, said kit comprising one or more enzymes having reverse transcriptase activity, wherein said enzymes are multi-subunit enzymes.
Dependencies are inferred from the claim text.