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AssigneeSyngenta Participations AG
InventorsJames Joseph Beck, Charles Jason Barnett, Michele S. Yarnall, Lillian Zeitouni
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The present invention relates to diagnostic assays for the identification of Acidovorax avenae subsp. citrulli, a bacterial pathogen of melons. In particular, the present invention relates to a novel protein that is specific for A. avenae subsp. citrulli, as well as antibodies specific thereof. The invention also relates to the use of primers in polymerase chain reaction (PCR) assays for the detection of Acidovorax avenae subsp. citrulli. The use of these primers and antibodies enables the detection of specific isolates of bacterial pathogens and the monitoring of disease development in plant populations.
A nucleic acid molecule encoding a 16S-23S spacer DNA sequences for the bacterial species Acidovorax avenae subsp. citrulli, Acidovorax avenae subsp. avenae, Xanthomonas curcurbitae, and Erwinia tracheiphila.
A nucleic acid molecule having sequence identity with at least 10 contiguous nucleotides of the 16S-23S rDNA spacer sequence from Acidovorax avenae subsp. citrulli.
A nucleic acid molecule comprising a nucleotide sequence of SEQ ID NOs:
A pair of oligonucleotide primers wherein at least one primer consists of the nucleotide sequence of SEQ ID NOS:
A pair of oligonucleotide primers comprising Aac-BITS10 (SEQ ID NO:
A method for the detection of a bacterial pathogen, comprising the steps of:
A method for the detection of a bacterial pathogen, comprising the steps of:
The method of claim 14, wherein at least one primer having the nucleotide sequence of SEQ ID NOS:
A diagnostic kit used in detecting a bacterial pathogen comprising at least one primer having at least 10 contiguous nucleotides of a 16S and 16S-23S rDNA spacer sequence of Acidovorax avenae subsp. citrulli.
A polypeptide comprising the amino acid sequence of DVVGAAPLTATNAAAA (SEQ ID NO:
Dependencies are inferred from the claim text.