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AssigneeNone recorded
InventorsMichifumi Tanga, Hiroshi Okamura, Kenichi Takagi, Kojiro Takahashi
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A support for fixing a nucleotide which contributes to the efficient clarification of DNA without damaging the terminal parts of the DNA and, therefore, is useful in the fields of biology, biochemistry and the like. This support for fixing a nucleotide is a chemically modified substrate on which oligonucleotide are immobilized characterized in that the oligonucleotide have a restriction enzyme cleavage site. This support is produced by chemically modifying the substrate, immobilizing a single-stranded oligonucleotide thereon, then hybridizing the single-stranded oligonucleotide with another single-stranded oligonucleotide having a base sequence complementary thereto, and ligating an oligonucleotide having a restriction enzyme site at the end thereof.
A support for fixing a nucleotide where an oligonucleotide is fixed on a chemically modified substrate, characterized in that, the said oligonucleotide has a restriction enzyme cleavage site.
A process for the production of a support for fixing a nucleotide where a substrate is chemically modified, a single-stranded oligonucleotide is fixed thereto and then another single-stranded oligonucleotide having a complementary base sequence to the above single-stranded oligonucleotide is hybridized to the said above single-stranded oligonucleotide whereupon the oligonucleotide having a restriction enzyme site at the terminal is bonded.
Dependencies are inferred from the claim text.
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Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate
Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses…
Enzymatic or biochemical coupling of nucleic acids to a solid phase: using probe arrays or probe chips
Indexing scheme relating to specific properties of organic compounds: compounds covalently bound to a solid support
Term end not estimated
The present invention relates to a support for fixing a nucleotide being able to fix nucleic acid, etc. useful in the field of molecular biology and the field related to genetic engineering and also to a process for producing the same.
Gene analysis is useful in the fields of molecular biology and genetic engineering and, in recent years, it has been utilized in the medical field such as finding of diseases as well.
In gene analysis, DNA chips have been developed in recent years and its analyzing velocity has become significantly quick. However, in the conventional DNA chips, there is used a method where a high-molecular substance such as polylysine is applied on the surface of a slide glass or a silicone substrate and, after that, DNA is fixed. There is another method where oligonucleotide is synthesized on a glass substrate using a semiconductor technique such as photolithograph.
(Preparation of a Support for Fixing of a Nucleotide Having EcoRI Cleavage Site)
(1) Chemical Modification of the Substrate
A substrate comprising diamond was irradiated with ultraviolet ray in chlorine gas so that the surface was chlorinated. After that, it was irradiated with ultraviolet ray in ammonia gas to aminate and then carboxylated by refluxing in chloroform using an acid chloride whereupon the substrate surface was chemically modified.