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US 2003/0190628 A1Published application
Beads, preparing method for the same, flow cytometer and program
AssigneeNone recorded
InventorsMotonao Nakao, Koichi Tatsuguchi
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AssigneeNone recorded
InventorsMotonao Nakao, Koichi Tatsuguchi
Estimating the term…
Disclosed are beads, which are chemically stable even at a normal temperature, are stable without being disintegrated even if being exposed to light, can emit a plurality of fluorescence excited by irradiating a light of single wavelength, and can be identified by a flow cytometer. A method for preparing the beads, a flow cytometer and a program for preparing the beads are also disclosed. By dyeing semiconductor nanoparticles as a fluorescent reagent to polystyrene beads, beads are identified. At the same time, semiconductor nanoparticles of a different fluorescence wavelength are also used for detection of reporter, enabling SNP specification of gene, monitoring or finding of the concentration of biopolymer such as a protein. The semiconductor nanoparticles have a feature that the fluorescence wavelength may vary by controlling the particle size and that they have a high durability compared to an ordinary fluorescent reagent since they are a semiconductor.
Beads, comprising:
A method of preparing beads, comprising the steps of:
A method of preparing beads, comprising the steps of:
A flow cytometer, comprising:
A program for allowing a computer to function as a flow cytometer, the flow cytometer comprising:
Dependencies are inferred from the claim text.
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No related US applications are recorded in the indexed filing history.
Backward citations are indexed for grants only.
Without spatial resolution of the texture or inner structure of the particle, e.g. processing of pulse signals: the analysis being performed on a…
The form being a particulate, a powder, an adsorbate, a bead or a sphere: the form being an inorganic particle, e.g. ceramic particles, silica…
Chemical, physical or physico-chemical processes in general; Their relevant apparatus: sequential or parallel reactions, e.g. for the synthesis of…
Hybridisation assays: characterised by the detection means
Term end not estimated
This application claims priority to Japanese Application Serial No. 2001-109940 filed Apr. 9, 2001.
1. Field of the Invention
The present invention relates to beads suitably used as a label of a probe or the like for detecting single nucleotide polymorphism (SNP), a method of preparing the beads, a flow cytometer for detecting the beads, and a program to be used for the flow cytometer.
FIG. 1 is a diagram showing a relation between mixing ratio of semiconductor nanoparticles and intensity of emitted fluorescence;
FIG. 2 is a schematic drawing showing a method for synthesizing DNA by parallel array method;
FIG. 3 is a diagram showing an example where a different processing is performed depending upon beads;
Recently, it has been proven that a semiconductor nanoparticles can be produced and that bonding of the semiconductor nanoparticles to a biopolymer is also applicable (Sience, vol. 281, p2013 (1998)). The semiconductor nanoparticles are inorganic compounds having a characteristic that, in its nature, a wavelength of fluorescence varies depending on their particles size.
In addition, since excitation is possible if an energy exceeding a band gap is given for excitation, ordinary semiconductor nanoparticles of various kinds of particle sizes can be excited by a laser outputting a high energy on a UV side.
Various methods have been reported for the method of preparing semiconductor nanoparticles, such as a vapor deposition method, an organic chemical synthesis method, and a preparation method using microwave (refer to the Publication of Japanese Patent No.3005683).