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OwnerJasmid Tanha, Ginette Dubuc +1inventors
InventorsJasmid Tanha, Ginette Dubuc, Saran Narang
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A phage display library of variable heavy domain (VHH or VH) fragments (sdAb fragments) derived from the antibody repertoire of a non-immunized llama is disclosed. The sdAb fragments of the library are characterized by the absence of cysteine residues in complementarity determining regions (CDRs) and a very low presence of residues of glutamic acid, arginine and glycine at positions 44, 45 and 47 respectively, of the VL interface of the variable heavy domain VHH. The large size of the library (in the order of 109) makes it a source of antigen-binding fragments having high affinity to almost any antigen of interest. The library is preferably generated using a modified fd-tet phage growing in plaques in the absence of a tetracycline.
A phage display library of antigen-binding fragments derived from llama antibodies, each antigen-binding fragment comprising at least a part of the variable heavy domain (VHH or VH) of a llama antibody.
An antigen-binding fragment derived from a llama antibody, said fragment comprising at least a part of the variable heavy domain (VHH or VH) of the antibody.
A cDNA library comprising nucleotide sequences coding for antigen-binding fragments of llama antibodies, said library obtained by performing the steps of:
A process for the preparation of an antigen-binding fragment of a llama antibody, said fragment binding to a predetermined antigen, said process comprising the steps of:
Dependencies are inferred from the claim text.