Glucuronofucan sulfate
AssigneeTakara Bio Inc.
InventorsTakeshiara Sakai, Kumiko Ishizuka, Kaoru Kojima, Kazuo Shimanaka, Katsushige Ikai, Ikunoshin Kato
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Abstract
Three enzymes degrading sulfated glucuronofucan; processes for producing these enzymes; activators for these enzymes; glucuronofucan sulfate and degradation products thereof; and a novel microorganism.
Claims22
A fucoidan deacetylase having the following chemical and physical properties:
- (I) acting on a sulfated glucuronofucan and a sulfated glucuronofucan oligosaccharide and hydrolyzing an acetyl group to release acetic acid;
- (II) having an optimal pH of about 6 to 9.1;
- and
- (III) having an optimal temperature of about 23 to 45° C.
An α-D-glucuronidase having the following chemical and physical properties:
- (I) acting on a deacetylated sulfated glucuronofucan and a sulfated glucuronofucan oligosaccharide and hydrolyzing an α-D-glucuronyl bond to release D-glucuronic acid;
- (II) having an optimal pH of about 5.8 to 7.8;
- and
- (III) having an optimal temperature of about 14 to 29° C.
An endo-α-L-fucosidase having the following chemical and physical properties:
- (I) acting on a deacetylated deglucuronylated sulfated glucuronofucan and a sulfated glucuronofucan oligosaccharide and hydrolyzing an a-L-fucosyl bond in an endo-type manner to generate an oligosaccharide having L-fucose at its reducing end;
- (II) having an optimal pH of about 4.5 to 7.5;
- and
- (III) having an optimal temperature of about 23 to 42° C.
A method for producing a sulfated glucuronofucan oligosaccharide, the method comprising:
- allowing a fucoidan deacetylase, an α-D-glucuronidase and an endo-α-L-fucosidase to act on a sulfated glucuronofucan;
- and
- collecting a sulfated glucuronofucan oligosaccharide.
A method for producing a sulfated glucuronofucan oligosaccharide, the method comprising:
- allowing an α-D-glucuronidase and an endo-α-L-fucosidase to act on a sulfated glucuronofucan deacetylated by alkali treatment;
- and
- collecting a sulfated glucuronofucan oligosaccharide.
A saccharide having a chemical structure of one selected from the group consisting of general formulas (I) to (III), or a salt thereof:
- 18 19
- wherein R is H, SO3H or CH3CO;
- n is 0 or an integer of 1 or more.
A sulfated glucuronofucan having the following chemical and physical properties, or a salt thereof:
- (1) containing fucose and glucuronic acid as constituting saccharides at a molar ratio of 35:10 to 44:10;
- and
- (2) containing a sulfated saccharide of general formula (VIII) as an essential component of the constituting saccharides:
- 20
A bacterium belonging to genus Fucophilus capable of utilizing plural sulfated polysaccharides derived from brown algae.
Dependencies are inferred from the claim text.