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US 2003/0186282 A1Published application
Using purified telomerase to identify telomerase activators and inhibitors
AssigneeGeron Corporation
InventorsScott L. Weinrich, Edward M. Atkinson III, Serge P. Lichtsteiner, Alain P. Vasserot, Ronald A. Pruzan
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Abstract
This invention provides purified telomerase and methods of purifying it. The methods involve the use of several sequential steps, including the use of matrices that bind molecules bearing negative charges, matrices that bind molecules bearing positive charges, intermediate-selectivity matrices, methods that separate molecules based on their size, shape, or buoyant density, and by affinity purification.
Claims25
A method to identify a regulator of telomerase activity, comprising:
- a) obtaining a preparation of mammalian telomerase enzyme purified by at least ˜2000-fold from a crude cell extract;
- b) combining the preparation with a compound;
- c) determining telomerase activity of the purified telomerase in the presence of the compound;
- d) identifying the compound as being a regulator of telomerase if the telomerase activity measured in step c) is affected by the presence of the compound.
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Dependencies are inferred from the claim text.