Avian pneumovirus vaccine
OwnerSagar M. Goyalinventor
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Abstract
Compositions and methods for ameliorating the clinical signs of an avian pneumovirus (APV) infection in a bird are disclosed. The compositions include immunologically effective amounts of an attenuated, cold-adapted APV. Methods for preparing an attenuated, cold-adapted APV composition are also described.
Claims45
A composition comprising an immunologically effective amount of an attenuated, cold-adapted avian pneumovirus (APV).
A method for preparing an attenuated, cold-adapted avian pneumovirus (APV) composition comprising
- a) inoculating a cell culture with an APV;
- and
- b) serially propagating said infected cell culture at successively lower temperatures until said APV becomes attenuated and cold-adapted.
A method for preparing an attenuated, cold-adapted avian pneumovirus (APV) composition, comprising the steps of:
- a) inoculating an avian cell culture with an APV;
- b) propagating said APV in said avian cell culture;
- c) inoculating a non-avian cell culture with an APV isolated from said propagated avian cell culture;
- d) serially propagating said non-avian infected cell culture at successively lower temperatures until said APV becomes attenuated and cold-adapted;
- and
- e) isolating said attenuated, cold-adapted APV from said non-avian infected cell culture.
A method for reducing the risk of an APV infection in a bird comprising inoculating a bird with an immunologically effective amount of an attenuated, cold-adapted APV composition.
An inoculated bird, wherein said bird contains an inoculant of an immunologically effective amount of an attenuated, cold-adapted APV.
Dependencies are inferred from the claim text.