Transcription factor stress-related polypeptides and methods of use in plants
AssigneeBASF Plant Science GmbH
InventorsRuoying Chen, Nocha Van Thielen, Oswaldo da Costa e Silva
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Abstract
A transgenic plant transformed by a Transcription Factor Stress-Related Protein (TFSRP) coding nucleic acid, wherein expression of the nucleic acid sequence in the plant results in increased tolerance to environmental stress as compared to a wild type variety of the plant. Also provided are agricultural products, including seeds, produced by the transgenic plants. Also provided are isolated TFSRPs, and isolated nucleic acid coding TFSRPs, and vectors and host cells containing the latter.
Claims41
An isolated nucleic acid, wherein the nucleic acid comprises a polynucleotide selected from the group consisting of:
- a) a polynucleotide as defined in SEQ ID NO:11, SEQ ID NO:2, SEQ ID NO:5, SEQ ID NO:8, SEQ ID NO:13, SEQ ID NO:15, or SEQ ID NO:17;
- b) a polynucleotide encoding a polypeptide as defined in SEQ ID NO:3, SEQ ID NO:6, SEQ ID NO:9, SEQ ID NO:12, SEQ ID NO:14, SEQ ID NO:16, or SEQ ID NO:18;
- and
- c) a polynucleotide complementary to a polynucleotide of any of a) or b) above.
An isolated nucleic acid, wherein the nucleic acid comprises a polynucleotide encoding a polypeptide having at least 76% sequence identity with a polypeptide as defined in SEQ ID NO:3, SEQ ID NO:6, SEQ ID NO:9, SEQ ID NO:12, SEQ ID NO:14, SEQ ID NO:16, or SEQ ID NO:18, and wherein the nucleic acid encodes a polypeptide that functions as a modulator of a plant stress response.
An isolated nucleic acid selected from the group consisting of a polynucleotide sequence encoding an LZ-2, an LZ-3, a DBF-2, and a DBF-3 from Physcomitrella patens;
- a BnDBF-1 from Brassica napus;
- and an OsDBF-1 and an OSDBF-2 from Oryza sativa, wherein the nucleic acid encodes a polypeptide that functions as a modulator of a plant stress response.
A first isolated nucleic acid that hybridizes under stringent conditions to a second nucleic acid selected from the group consisting of:
- a) a second nucleic acid comprising a polynucleotide as defined in SEQ ID NO:2, SEQ ID NO:5, SEQ ID NO:8, SEQ ID NO:11, SEQ ID NO:13, SEQ ID NO:15, or SEQ ID NO:17;
- and
- b) a second nucleic acid encoding a polypeptide as defined in SEQ ID NO:3, SEQ ID NO:6, SEQ ID NO:9, SEQ ID NO:12, SEQ ID NO:14, SEQ ID NO:16, or SEQ ID NO:18.
A method of producing a transgenic plant containing a nucleic acid wherein the plant has an increased tolerance to an environmental stress as compared to a wild type variety of the plant comprising, transforming a plant cell with an expression vector comprising the nucleic acid and generating from the plant cell the transgenic plant, wherein the nucleic acid encodes a polypeptide that functions as a modulator of a plant stress response, and wherein the nucleic acid is selected from the group consisting of:
- a) a polynucleotide as defined in SEQ ID NO:2, SEQ ID NO:5, SEQ ID NO:8, SEQ ID NO:11, SEQ ID NO:13, SEQ ID NO:15, or SEQ ID NO:17;
- b) a polynucleotide encoding a polypeptide as defined in SEQ ID NO:3, SEQ ID NO:6, SEQ ID NO:9, SEQ ID NO:12, SEQ ID NO:14, SEQ ID NO:16, or SEQ ID NO:18;
- c) a polynucleotide encoding a polypeptide having at least 76% sequence identity with a polypeptide as defined in SEQ ID NO:3, SEQ ID NO:6, SEQ ID NO:9, SEQ ID NO:12, SEQ ID NO:14, SEQ ID NO:16, or SEQ ID NO:18;
- d) a polynucleotide comprising at least 60 consecutive nucleotides of any of a) or b) above;
- and
- e) a polynucleotide complementary to a polynucleotide of any of a) through d) above.
Dependencies are inferred from the claim text.