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US 2003/0172406 A1Published application
Methods for increasing Biomass of transgenic maize
AssigneeProdiGene, Inc.
InventorMichael E. Horn
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Abstract
A method of improving seed set of transgenic maize plants and improving agronomic characteristics of such plants is disclosed which employs the use of maize host cells that are the result of a cross between one parent that is Stiff Stalk derived germplasm and a second parent that is from the HiII genotype.
Claims15
A method of Agrobacterium mediated maize transformation comprising:
- providing for transformation an hybrid plant cell, said cell being the result of a cross between a first parent which is derived from a Stiff Stalk germplasm pool and a second parent which is a HiII genotype, and
- contacting said hybrid embryo with an Agrobacterium based vector under transformation conditions.
A method of a producing heterologous protein in a maize plant cell comprising:
- providing a hybrid maize cell for transformation, said hybrid cell being the result of a cross between a first parent which is derived from a Stiff Stalk germplasm pool and a second parent which is a HiII genotype, and
- introducing to said maize cell an Agrobacterium-based vector comprising a nucleotide sequence encoding said heterologous protein.
A method of increasing transformation frequency of corn plants comprising:
- selecting a plant which is HiII genotype;
- selecting a plant derived from Stiff Stalk germplasm;
- crossing the HiII and Stiff Stalk plant to produce progeny plant tissue;
- contacting the progeny plant tissue with an Agrobacterium vector under transformation conditions such that transformation frequency of the progeny is increased.
1 more element
A method of Agrobacterium mediated maize transformation comprising:
- providing for transformation an hybrid plant cell, said cell being the result of a cross between a first parent which is derived from Lancaster germplasm pool and a second parent which is a HiII genotype, and
- contacting said hybrid embryo with an Agrobacterium based vector under transformation conditions.
Dependencies are inferred from the claim text.