Method of modifying plant characters by the targeted expression of a cell cycle control protein
OwnerPeter Crook Lloyd John, Kerong Zhang +2inventors
InventorsPeter Crook Lloyd John, Kerong Zhang, Francis John Sek, Wim Van Camp
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Abstract
This invention provides a method for modifying plant growth and development comprising expressing an isolated nucleic acid sequence encoding Cdc25 or a homologue, analogue or derivative thereof in one or more plant cells, tissues or organs operably under the control of a regulatable promoter that does not confer expression throughout the plant under all conditions.
Claims58
A method of modifying a plant character selected from the group consisting of a morphological character, a biochemical character, and a physiological character comprising expressing in a particular cell, tissue or organ of a plant an isolated gene encoding a Cdc25 protein or a homologue, analogue or derivative thereof operably under the control of a regulatable promoter sequence that is operable in a plant or a cell, tissue or organ thereof subject to the proviso that the modified plant character is not an enhancement of lateral root production.
The method according to claim 40 wherein the modified plant character comprises enhanced endoreplication or enhanced endoreduplication.
The method according to claim 49, wherein culturing of the plant cell under conditions sufficient for transcription and translation to occur includes organogenesis or embryogenesis.
A gene construct comprising an isolated gene encoding a Cdc25 protein or a homologue, analogue or derivative of Cdc25, placed operably in connection with a regulatable promoter sequence that is operable in a plant or a cell, tissue or organ of said plant, wherein said regulatable promoter sequence is selected from the group consisting of:
- (i) a dexamethasone-inducible promoter sequence;
- (ii) a patatin gene promoter sequence;
- (iii) a modified patatin gene promoter sequence having a deletion in a sucrose-responsive element;
- (iv) an auxin-inducible SAUR gene promoter sequence;
- (v) a rolB gene promoter sequence;
- (vi) a rice prolamin NRP33 gene promoter sequence;
- (vii) a synthetic promoter sequence comprising an endosperm box motif of the barley Hor2 gene;
- (viii) a LEAFY gene promoter sequence;
- (ix) a knat1 gene promoter sequence;
- (x) a kn1 gene promoter sequence;
- (xi) a CLAVATA1 gene promoter sequence;
- (xii) a cab-6 gene promoter sequence;
- (xiii) a rice REB gene promoter sequence;
- and (xiv) a ubi7 gene promoter sequence.
Dependencies are inferred from the claim text.