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US 2003/0172398 A1Published application
Novel delta-12 desaturase and methods of using it for synthesis of polyunsaturated fatty acids
OwnerJohn A. Browseinventor
Life of this patent
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Abstract
Desaturase enzymes, and especially animal Δ12-desaturases, and the use of such enzymes to alter fatty acid saturation, especially fatty acid saturation in oilseeds, are disclosed. Also disclosed are nucleic acid sequences encoding animal Δ12-desaturase enzymes.
Claims32
A purified desaturase protein, comprising an amino acid sequence selected from the group consisting of:
- (a) an amino acid sequence as shown in SEQ. ID NO. 2;
- (b) an amino acid sequence that differs from that specified in (a) by one or more conservative amino acid substitutions;
- (c) an amino acid sequences having at least 60% sequence identity to the sequences specified in (a) or (b);
- and
- (d) fragments of (a), (b), or (c),
- wherein the purified protein has desaturase activity.
3 more elements
An isolated nucleic acid molecule that:
- (a) hybridizes under low-stringency conditions with a nucleic acid probe, the probe comprising a sequence as shown in SEQ ID NO:
- 1, and fragments thereof, and
- (b) encodes a protein having desaturase activity.
An isolated nucleic acid molecule that:
- (a) has at least 60% sequence identity with a nucleic acid sequence as shown in SEQ ID NO:
- 1;
- and
- (b) encodes a protein having desaturase activity.
1 more element
A method for identifying a nucleic acid sequence, comprising:
- (a) hybridizing the nucleic acid sequence to at least 10 contiguous nucleotides of a sequence as shown in SEQ ID NO:
- 1;
- and
- (b) identifying the nucleic acid sequence as corresponding to a nucleic acid encoding a desaturase.
1 more element
Dependencies are inferred from the claim text.