Transgenic plants having resistance to a fungal disease
OwnerYigal Cohen, David Kenigsbuchinventors
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Abstract
The present invention provides novel nucleic acid sequences, which upon their transformation into plants, provide the later with resistance to plant fungal diseases, in particular to downy mildew disease. The nucleic acids of the invention, one or more thereof, are transformed into a plant cell, from which said plant is generated. Such plants also form part of the invention. The nucleic acid of the invention may also be used for mass production of biologically functional proteins or peptides encoded thereby.
Claims27
A nucleic acid encoding a serine-glyoxylate aminotransferase (SGT) or an alanine-glyoxylate aminotransferase (AGT) for use in the production of a plant expressing said nucleic acid, said plant having, as a result of said expression, resistance to a disease caused by Pseudoperonospora cubensis or by Peronospora tabacina.
The nucleic acid of any one of the preceding claim comprising a sequence selected from:
- (i) a nucleic acid sequence substantially set forth in SEQ ID NO:1, SEQ ID NO:2, SEQ ID NO:39 or SEQ ID NO:40;
- (ii) a nucleic acid sequence having at least 90% identity with the sequence set forth in SEQ ID NO:1, SEQ ID NO:2, SEQ ID NO:39 or SEQ ID NO:40 or with a region thereof;
- (iii) biologically functional fragments of the sequence of (a) or (b).
A construct comprising a nucleic acid encoding a serine-glyoxylate aminotransferase (SGT) or an alanine-glyoxylate aminotransferase (AGT), which when transformed into a plant cell, said nucleic acid is expressed and the plant cell has, following said transformation, resistance to a disease caused by Pseudoperonospora cubensis or by Peronospora tabacina.
A plant comprising a cell transformed with a nucleic acid encoding a serine-glyoxylate aminotransferase (SGT) or an alanine-glyoxylate aminotransferase (AGT), the plant cell expressing said nucleic acid and having resistance to a disease caused by Pseudoperonospora cubensis or by Peronospora tabacina.
A method of producing a transgenic plant having resistance to a disease caused by Pseudoperonospora cubensis or by Peronospora tabacina said method comprises:
- (i) providing a plant cell not resistant to said disease;
- (ii) introducing into said plant cell a nucleic acid encoding a serine-glyoxylate aminotransferase (SGT) or an alanine-glyoxylate aminotransferase (AGT), or a contract comprising a nucleic acid encoding a SGT or an AGT operatively linked to at least one control element for expressing said nucleic acid in said plant cell;
- (iii) regenerating from said plant cell a plant having, following said transformation, resistance to said disease.
The method of claim 16, wherein said plant is a transgene from Citrullus lanatus, Cucurbita moschata, Cucurbita pepo, Luffa sp., Lagenaria sp., Momordica sp., or Cucumis melo.
A nucleic acid selected from:
- (i) a nucleic acid sequence substantially set forth in SEQ ID NO:1, SEQ ID NO:2, SEQ ID NO:39 or SEQ ID NO:40;
- (ii) a nucleic acid sequence having at least 90% identity with the sequence set forth in SEQ ID NO:1, SEQ ID NO:2, SEQ ID NO:39 or SEQ ID NO:40 or with a region thereof;
- (iii) biologically functional fragments of the sequence of (a) or (b).
The nucleic acid of claim 20, being a DNA or and RNA.
A host cell transformed with the nucleic acid of any one of claims 23 to 25.
Dependencies are inferred from the claim text.