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AssigneePharmacia Corporation
InventorsTanja Ball, Susanne Vrtala, Wolfgang Sperr, Peter Valent, Markus Susani, Dietrich Kraft, Rudolf Valenta, Sylvia Laffer
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A recombinant DNA molecule comprising a nucleotide sequence (I) which codes for a polypeptide displaying the antigenicity of one, two or more of the Phl p I epitope clones (28, 34, 41, 42, 43, 50, 52, 64, 80, 85, 86, 95, 97, 98, 103, 108, 109, 113, 114) with the amino acid sequences defined in FIG. 2 and preferably being derived from grasses or monocotyledonic plants, or a nucleotide sequence (II) which hybridizes with such a nucleotide sequence (I) under conditions of high stringency. Polypeptides displaying the antigenicity of one, two or more of the Phl p I epitope clones (28, 34, 41, 42, 43, 50, 52, 64, 80, 85, 86. 95, 97, 98, 103, 108, 109, 113, 114) with the amino acid sequences defined in FIG. 2. Recombinant expression vectors containing the recombinant molecule and host cells transformed with the vector. Diagnostic methods based on utilizing the polypeptides in immunoassays for humoral antibodies and cellular reactions.
A recombinant DNA molecule comprising a nucleotide sequence (I) which codes for a polypeptide displaying the antigenicity of one, two or more of the Phl p I epitope clones 28, 34, 41, 42, 43, 50, 52, 64, 80, 85, 86, 95, 97, 98, 103, 108, 109, 113, 114 with the amino acid sequences defined in FIG. 2 and preferably being derived from grasses or monocotyledonic plants, or a nucleotide sequence (II) which hybridizes with such a nucleotide sequence (I) under conditions of high stringency.
A recombinant DNA molecule according to any one of claims 1-2, comprising a nucleotide sequence which codes for a polypeptide having antigenic crossreactivity and a high degree of homology, preferably >50% such as >60% or >75%, with Phl p I epitopes from grasses or other monocotyledonic plants, preferably those defined by the amino acid sequences given in FIG. 2 for clones 28, 34, 41, 42, 43, 50, 52, 64, 80, 85, 86, 95, 97, 98, 103, 108, 109, 113, 114.
A recombinant expression vector in which an expression control sequence is operationally linked to a coding nucleotide sequence which hybridizes with a Phl p I cDNA clone defined by the amino acids sequences given in FIG. 2, said hybridization taking place under conditions of high stringency.
A polypeptide displaying the antigenicity of at least one of the Phl p I epitopes defined by the amino acid sequences given in clones 28, 34, 41, 42, 43, 50, 52, 64, 80, 85, 86, 95, 97, 98, 103, 108, 109, 113, 114 of FIG. 2 or a Phl p I unique portion of these sequences.
Dependencies are inferred from the claim text.