Regulators of bacterial virulence factor expression
OwnerPeter J. McNamarainventor
Life of this patent
Estimating the term…
Abstract
Genetic loci that encode polypeptides that regulate the expression of virulence factors in bacteria, particularly S. aureus. Methods of detecting the gene or gene products for purposes of detecting S. aureus or diagnosing a patient suspected of being infected by S. aureus.
Claims51
An isolated polynucleotide comprising a member selected from the group consisting of:
- (a) a polynucleotide of SEQ ID NO:1, SEQ ID NO:3, or SEQ ID NO:5 or a complement of SEQ ID NO:1, SEQ ID NO:3, or SEQ ID NO:5;
- (b) a fragment of the polynucleotide of SEQ ID NO:1, SEQ ID NO:3, or SEQ ID NO:5 wherein the fragment comprises at least 20 contiguous nucleotides of SEQ ID NO:1, SEQ ID NO:3, or SEQ ID NO:5;
- (c) a polynucleotide having at least 70% sequence identity to the sequence of SEQ ID NO:1, SEQ ID NO:3, or SEQ ID NO:5;
- (d) a polynucleotide that hybridizes to any one of the polynucleotides of (a), (b) or (c) under conditions of 5× SSC, 50% formamide and 42° C., and which encodes a protein having the same biological function;
- and
- (e) a polynucleotide encoding the same amino acid sequence as any of the polynucleotides of (a), (b), (c) or (d) but which exhibits regular degeneracy in accordance with the degeneracy of the genetic code.
A recombinant polynucleotide comprising a member selected from the group consisting of:
- (a) a polynucleotide of SEQ ID NO:1, SEQ ID NO:3, or SEQ ID NO:5 or a complement of SEQ ID NO:1, SEQ ID NO:3, or SEQ ID NO:5;
- (b) a fragment of the polynucleotide of SEQ ID NO:1, SEQ ID NO:3, or SEQ ID NO:5 wherein the fragment comprises at least 20 contiguous nucleotides of SEQ ID NO:1, SEQ ID NO:3, or SEQ ID NO:5;
- (c) a polynucleotide having at least 70% sequence identity to the sequence of SEQ ID NO:1, SEQ ID NO:3, or SEQ ID NO:5;
- (d) a polynucleotide that hybridizes to any of the polynucleotides of (a), (b) or (c) under conditions of 5× SSC, 50% formamide and 42° C., and which encodes a protein having the same biological function;
- and
- (e) a polynucleotide encoding the same amino acid sequence as any of the polynucleotides of (a), (b), (c) or (d) but which exhibits regular degeneracy in accordance with the degeneracy of the genetic code.
A protein or polypeptide encoded by the polynucleotide selected from the group consisting of:
- (a) a polynucleotide of SEQ ID NO:1, SEQ ID NO:3, or SEQ ID NO:5 or a complement of SEQ ID NO:1, SEQ ID NO:3, or SEQ ID NO:5;
- (b) a fragment of the polynucleotide of SEQ ID NO:1, SEQ ID NO:3, or SEQ ID NO:5 wherein the fragment comprises at least 20 contiguous nucleotides of SEQ ID NO:1, SEQ ID NO:3, or SEQ ID NO:5;
- (c) a polynucleotide having at least 70% sequence identity to the sequence of SEQ ID NO:1, SEQ ID NO:3, or SEQ ID NO:5;
- (d) a polynucleotide that hybridizes to any of the polynucleotides of (a), (b) or (c) under conditions of 5× SSC, 50% formamide and 42° C., and which encodes a protein having the same biological function;
- and
- (e) a polynucleotide encoding the same amino acid sequence as any of the polynucleotides of (a), (b), (c) or (d) but which exhibits regular degeneracy in accordance with the degeneracy of the genetic code.
An isolated protein or polypeptide comprising the amino acid sequence of SEQ ID NO:2.
A recombinant protein or polypeptide comprising the amino acid sequence of SEQ ID NO:2.
An isolated protein or polypeptide comprising a Rot polypeptide sequence from Staphylococcus aureus, wherein the polypeptide comprises at least 15 contiguous amino acids of SEQ ID NO:2.
An isolated protein or polypeptide comprising the amino acid sequence as depicted in SEQ ID NO:4.
A recombinant protein or polypeptide comprising the amino acid sequence as depicted in SEQ ID NO:4.
An isolated protein or polypeptide comprising an Rlp polypeptide sequence from Staphylococcus aureus, wherein the protein or polypeptide comprises at least 15 contiguous amino acids of SEQ ID NO:4.
Dependencies are inferred from the claim text.