Genes and proteins for the biosynthesis of polyketides
OwnerChris M. Farnet, Emmanuel Zazopoulos +2inventors
InventorsChris M. Farnet, Emmanuel Zazopoulos, Alfredo Staffa, Xianshu Yang
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Abstract
Genes and proteins involved in the biosynthesis of polyketides by microorganisms, including the genes and proteins forming the biosynthetic loci for the polyketide dorrigocin from Streptomyces platensis subsp. rosaceus and the polyketide lactimidomycin from Streptomyces amphibiosporus. The genes and proteins allow direct manipulation of dorrigocin, lactimidomycin and related chemical structures via chemical engineering of the enzymes involved in the biosynthesis of dorrigocin and lactimidomycin.
Claims37
An isolated, purified or enriched nucleic acid comprising a sequence selected from the group consisting of SEQ ID NOS:
- 1, 22 and 25;
- the sequences complementary to SEQ ID NOS:
- 1, 22 and 25;
- fragments comprising at least 10 consecutive nucleotides of SEQ ID NOS:
- 1, 22 and 25;
- and fragments comprising at least 10 consecutive nucleotides of the sequences complementary to SEQ ID NOS:
- 1, 22 and 25.
An isolated, purified or enriched nucleic acid comprising a sequence selected from the group consisting of SEQ ID NOS:
- 3, 5, 7, 9, 11, 13, 15, 17, 19, 21, 24, 27, 29, 31, 33, 35, 37, 39, 41, 43 and the sequences complementary thereto.
An isolated, purified or enriched nucleic acid comprising at least 10 consecutive bases of a sequence selected from the group consisting of SEQ ID NOS:
- 3, 5, 7, 9, 11, 13, 15, 17, 19, 21, 24, 27, 29, 31, 33, 35, 37, 39, 41, 43 and the sequences complementary thereto.
An isolated or purified polypeptide comprising a sequence selected from the group consisting of SEQ ID NOS:
- 2, 4, 6, 8, 10, 12, 14, 16, 18, 20, 23, 26, 28, 30, 32, 34, 36, 38, 40 and 42.
An isolated or purified antibody capable of specifically binding to a polypeptide having a sequence selected from the group consisting of SEQ ID NOS:
- 2, 4, 6, 8, 10, 12, 14, 16, 18, 20, 23, 26, 28, 30, 32, 34, 36, 38, 40 and 42.
An isolated or purified antibody capable of specifically binding to a polypeptide comprising at least 10 consecutive amino acids of one of the polypeptides of SEQ ID NOS:
- 2, 4, 6, 8, 10, 12, 14, 16, 18, 20, 23, 26, 28, 30, 32, 34, 36, 38, 40 and 42.
A method of making a polypeptide having a sequence selected from the group consisting of SEQ ID NOS:
- 2, 4, 6, 8, 10, 12, 14, 16, 18, 20, 23, 26, 28, 30, 32, 34, 36, 38, 40 and 42 comprising introducing a nucleic acid encoding said polypeptide, said nucleic acid being operably linked to a promoter, into a host cell.
A method of making a polypeptide having at least 10 consecutive amino acids of a sequence selected from the group consisting of SEQ ID NOS:
- 2, 4, 6, 8, 10, 12, 14, 16, 18, 20, 23, 26, 28, 30, 32, 34, 36, 38, 40 and 42 comprising introducing a nucleic acid encoding said polypeptide, said nucleic acid being operably linked to a promoter, into a host cell.
A computer readable medium having stored thereon a sequence selected from the group consisting of a nucleic acid code of SEQ ID NOS:
- 1, 3, 5, 7, 9, 11, 13, 15, 17, 19, 21, 22, 24, 25, 27, 29, 31, 33, 35, 37, 39, 41, and 43 and a polypeptide code of SEQ ID NOS:
- 2, 4, 6, 8, 10, 12, 14, 16, 18, 20, 23, 26, 28, 30, 32, 34, 36, 38, 40 and 42.
A computer system comprising a processor and a data storage device wherein said data storage device has stored thereon a sequence selected from the group consisting of a nucleic acid code of SEQ ID NOS:
- 1, 3, 5, 7, 9, 11, 13, 15, 17, 19, 21, 22, 24, 25, 27, 29, 31, 33, 35, 37, 39, 41, and 43 and a polypeptide code of SEQ ID NOS:
- 2, 4, 6, 8, 10, 12, 14, 16, 18, 20, 23, 26, 28, 30, 32, 34, 36, 38, 40 and 42.
An isolated gene cluster comprising open reading frames encoding polypeptides sufficient to direct the synthesis of a dorrigocin compound or analogue.
An isolated gene cluster comprising open reading frames encoding polypeptides sufficient to direct the synthesis of a lactimidomycin or a lactimidomycin analogue.
Dependencies are inferred from the claim text.