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US 2003/0171560 A1Published application
Purification of protein inclusion bodies by crossflow microfiltration
AssigneeBayer Aktiengesellschaft
InventorJorg Peters
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AssigneeBayer Aktiengesellschaft
InventorJorg Peters
Estimating the term…
The invention relates to a process for purifying and/or concentrating inclusion bodies (refractile bodies) in a highly concentrated particle-containing solution, in which process the inclusion body-containing suspension is directed tangentially past one or more semipermeable membranes, so that the inclusion bodies are retained behind the membranes and that substances having a relatively small molecular weight may pass through the membrane and/or be adsorbed to the membrane, and a purified and/or concentrated inclusion body suspension is obtained. In the same manner, cell wall particles are removed from a inclusion body suspension. The invention further relates to the use of a crossflow microfiltration unit for purifying and/or concentrating inclusion bodies in a suspension and for removing cell wall particles from a inclusion body preparation.
Process for purifying and/or concentrating inclusion bodies in a solution, which is characterized in that the inclusion body-containing solution is directed tangentially past one or more semipermeable membranes so that the membranes retain the inclusion bodies and that substances or components having a relatively small molecular weight or particle diameter can pass through, thus obtaining a purified and/or concentrated inclusion body solution, and on the semipermeable membrane a wide-meshed matrix is located which is constructed of intersecting longitudinal and transverse filaments such that the adjacent longitudinal and transverse filaments are, in each case, at a distance from one another which is 5 to 15 times greater than their thickness which is in the range from 150 to 600 μm.
Dependencies are inferred from the claim text.