Polymer composition for forming surface of biosensor
AssigneeToudai TLO, Ltd.
InventorsKazunori Kataoka, Yukio Nagasaki, Hidenori Otsuka, Mitsuhiro Kaneko
Life of this patent
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Abstract
A polymer composition is provided, which contains a polymer or copolymer having a mercapto group at one end and functional group or ligand at the other end and also having a polyethylene glycol segment. Such a composition can form a biosensor surface of reduced non-specific adsorbability for proteins and the like.
Claims8
A polymer composition for forming surface of a biosensor utilizing surface plasmon resonance (SPR), which comprises a polymer represented by the following general formula (I) as an active ingredient, solvent and optionally an adjuvant or adjuvants:
- HS—L1—(B)m—L2—(CH2CH2O)n—L3—X (I)
- in which L1, L2 and L3 each represents a valence bond or linker independently of each other, but where m is zero (0), L1 and L2 may together form a valence bond or a linker;
- B stands for a formula,
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- where R1 and R2 each independently stands for hydrogen atom or C1-5 alkyl group and p is an integer of 2-5;
- x stands for a hydrogen atom, functional group or ligand;
- m is an integer of 0-10,000, and
- n is an integer of 10-20,000.
Biosensor chips for detecting surface plasmon resonance (SPR), in which a polymer represented by the following general formula (I) is absorbed (or adsorbed) onto the sensor chip surfaces via the mercapto group present at one end of said polymer:
- general formula (I)
- HS—L1—(B)m—L2—(CH2CH2O)n—L3—X (I)
- in which L1, L2 and L3 represent valence bonds or linkers independently of each other, but where m is zero (0), L1 and L2 may together form a valence bond or a linker;
- B stands for a formula,
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- where R1 and R2 each independently stands for hydrogen atom or C1-5 alkyl group and p is an integer of 2-5;
- x stands for a hydrogen atom, functional group or ligand;
- m is an integer of 0-10,000, and
- n is an integer of 10-20,000.
Use of a polymer represented by the following general formula (I), for forming surfaces of biosensor chips utilizing surface plasmon resonance (SPR):
- general formula (I)
- HS—L1—(B)m—L2—(CH2CH2O)n—L3—X (I)
- in which L1, L2 and L3 represent valence bonds or linkers independently of each other, but where m is zero (0), L1 and L2 may together form a valence bond or a linker;
- B stands for a formula,
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- where R1 and R2 each independently stands for hydrogen atom or C1-5 alkyl group and p is an integer of 2-5;
- x stands for a hydrogen atom, functional group or ligand;
- m is an integer of 0-10,000, and
- n is an integer of 10-20,000.
A method for making biosensor chips utilizing surface plasmon resonance (SPR), which comprises contacting a solution or dispersion of a polymer represented by the following general formula (I):
- HS—L1—(B)m—L2—(CH2CH2O)n—L3—X (I)
- in which L1, L2 and L3 represent valence bonds or linkers independently of each other, but where m is zero (0), L1 and L2 may together form a valence bond or a linker;
- B stands for a formula,
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- where R1 and R2 each independently stands for hydrogen atom or C1-5 alkyl group and p is an integer of 2-5;
- x stands for a hydrogen atom, functional group or ligand;
- m is an integer of 0-10,000, and
- n is an integer of 10-20,000
- with surfaces of sensor chips, said surfaces being formed of at least one metal selected from the group consisting of gold, silver, platinum and aluminum, whereby causing the polymer to be chemically adsorbed onto or chemically absorbed into said surfaces via the mercapto group present at one end of said polymer, and if necessary covalently bonding a ligand thereto via the functional group X.
Dependencies are inferred from the claim text.