Peptides blocking vascular endothelial growth factor (VEGF)-mediated angiogenesis, polynucleotides encoding said peptides and methods of use thereof
AssigneeInstitut Pasteur
InventorsRoselyne Tournaire, Caroline Demangel, Claude Derbin, Gerard Perret, Jean-Claude Mazie, Jean Plouet, Roger Vassy
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Abstract
The present invention provides peptides which can interact with VEGF and inhibit VEGF interaction with KDR or anti-VEGF antibody thereby inhibiting VEGF mediated angiogenesis or angiogeneis related diseases, polynucleotide encoding the peptides, vectors containing the polynucleotides, pharmaceutical compositions containing the peptides, and methods of inhibiting angiogenesis with the peptides.
Claims37
A method of screening for peptide molecules capable interacting with VEGF comprising:
- generating a random peptide library;
- contacting said library to a ligand capable of binding VEGF;
- selecting the molecules from said library which bind to said ligand.
A purified peptide selected from the group consisting of SEQ ID NO:8, SEQ ID NO:9, SEQ ID NO:10, SEQ ID NO:11, SEQ ID NO:12, SEQ ID NO:13, SEQ ID NO:14;
- wherein said peptide inhibits the binding of VEGF to KDR.
A purified peptide selected from the group consisting of SEQ ID NO:1, SEQ ID NO:2, SEQ ID NO:3, SEQ ID NO:4, SEQ ID NO:5, SEQ ID NO:6, SEQ ID NO:7;
- wherein said peptide inhibits the binding of anti-VEGF antibody to VEGF.
A purified peptide of the sequence ATWLPPR (SEQ ID NO:
- 1).
Dependencies are inferred from the claim text.