Alpha-amylase mutants

A variant of a parent Termamyl-like α-amylase, which variant has α-amylase activity and exhibits an alteration in at least one of the following properties relative to said parent α-amylase:

• substrate specificity, substrate binding, substrate cleavage pattern, thermal stability, pH/activity profile, pH/stability profile, stability towards oxidation, Ca2+ dependency and specific activity;
• said variant comprising one or more mutations corresponding to one or more of the following mutations in the amino acid sequence shown in SEQ ID No. 2:
• the mutations:
• A181E,D,Q,N,V;
• I201 (bulkier amino acid), including I201W,F,L;
• Y203Q;
• Q9K,L,E;
• F11R,K,E;
• E12Q;
• D100N,L;
• V101H,R,K,D,E,F;
• V102A,T;
• I103H,K;
• N104R,K,D;
• H105R,K,D,E,W,F;
• L196R,K,D,E,F,Y;
• I212R,K,D,E;
• L230H,K,I;
• A232G,H,F,S,V;
• V233D;
• K234L,E;
• I236R,K,N,H,D,E;
• L241R,K,D,E,F;
• A260S;
• W263H;
• Q264R,D,K,E;
• N265K,R,D;
• A269R,K,D,E;
• L270R,K,H,D,E;
• V283H,D;
• F284H;
• D285N,L;
• V286R,K,H,D,E;
• Y290R,E,K;
• V312R,K,D,E;
• F323H;
• D325N;
• N326K,H,D,L;
• H327Q,N,E,D,F;
• Q330L,E;
• G332D;
• Q333R,K,H,E,L;
• S334A,V,T,L,I,D;
• L335G,A,S,T,N;
• E336R+R375E;
• T337D,K;
• T338D,E;
• T339D;
• Q360K,R,E;
• D365N;
• or G371D,R;
• substitutions at positions H68, H91, H247, R305, K306, H382, K389, H405, H406, H450 or R483;
• the mutations:
• H140Y;
• H142Y;
• H159Y;
• H140D+H142R;
• H140K+H142D;
• or H142Y+H156Y;
• deletion of three amino acids within the partial sequence from T369 to I377, including the deletions:
• K370*+G371*+D372* or D372*+S373*+Q374*;
• replacement of the partial amino acid sequence from T369 to I377 with one of the following sequences (sequence number increasing from left to right):
• I-P-T-H-S-V;
• I-P-T-H-G-V;
• I-P-Q-Y-N-I;
• substitutions at positions R169 or R173, including R169I,L,F,T or R173I,L,F,T;
• the mutations H156D;
• I201F;
• I212F;
• A209L,T;
• or V208I;
• substitutions at positions N172, A181, N188, N190, H205, D207, A209, A210, E211, Q264 or N265, including N172R,H,K;
• A181T;
• N188P;
• N190L,F;
• H205C;
• D207Y;
• A209L,T,V;
• A210S;
• E211Q;
• Q264A,E,L,K,S,T;
• N265A,S,T,Y;
• or Q264S+N265Y;
• the mutations:
• H156Y+A181T+A209V;
• H156Y+A181T+N190F+A209V+Q264S;
• A1*+N2*+L3V+M15T+R23K+S29A+A30E+Y31H+A33S+E34D+H35I+H156Y+A181T+A209V;
• A1*+N2*+L3V+M15T+R23K+S29A+A30E+Y31H+A33S+E34D+H35I+H156Y+A181T+N190F+A209V;
• or
• A1*+N2*+L3V+M15T+R23K+S29A+A30E+Y31H+A33S+E34D+H35I+H156Y+A181T+N190F+A209V+Q264S.