Subtilase enzymes

A modified subtilase comprising a mutation in an amino acid sequence of a subtilase, wherein the mutation is an insertion of at least one additional amino acid residue at position 96 of the active site loop (b) region corresponding to positions 95 to 103, wherein the positions are numbered according to the amino acid sequence of the mature subtilisin BPN′.

A modified subtilase comprising a mutation in an amino acid sequence of a subtilase, wherein the mutation is an insertion of at least one additional amino acid residue at position 97 of the active site loop (b) region corresponding to positions 95 to 103, wherein the positions are numbered according to the amino acid sequence of the mature subtilisin BPN′.

The modified subtilase of claim 59, wherein the mutation is an insertion of two or more amino acid residues at position 97.

A modified subtilase comprising a mutation in an amino acid sequence of a subtilase, wherein the mutation results in the modified subtilase having, relative to the subtilase, one or more additional amino acid residues at position 98 of the active site loop (b) region corresponding to positions 95 to 103, wherein the positions are numbered according to the amino acid sequence of the mature subtilisin BPN′.

A modified subtilase comprising a mutation in an amino acid sequence of a subtilase, wherein the mutation is an insertion of at least one additional amino acid residue at position 99 of the active site loop (b) region corresponding to positions 95 to 103, wherein the positions are numbered according to the amino acid sequence of the mature subtilisin BPN′.

A modified subtilase comprising a mutation in an amino acid sequence of a subtilase, wherein the mutation is an insertion of at least one additional amino acid residue at position 100 of the active site loop (b) region corresponding to positions 95 to 103, wherein the positions are numbered according to the amino acid sequence of the mature subtilisin BPN′.

A modified subtilase comprising a mutation in an amino acid sequence of a subtilase, wherein the mutation is an insertion of at least one additional amino acid residue at position 101 of the active site loop (b) region corresponding to positions 95 to 103, wherein the positions are numbered according to the amino acid sequence of the mature subtilisin BPN′.

A modified subtilase comprising a mutation in an amino acid sequence of a subtilase, wherein the mutation is an insertion of at least one additional amino acid residue at position 102 of the active site loop (b) region corresponding to positions 95 to 103, numbered according to the amino acid sequence of the mature subtilisin BPN′.

A modified subtilase comprising a mutation in an amino acid sequence of a subtilase, wherein the mutation is an insertion of at least one additional amino acid residue at position 103 of the active site loop (b) region corresponding to positions 95 to 103, wherein the positions are numbered according to the amino acid sequence of the mature subtilisin BPN′.

A subtilase comprising at least nine amino acid residues in the active site loop (b) region corresponding to positions 95 to 103, wherein the positions are numbered according to the amino acid sequence of the mature subtilisin BPN′.

A subtilase belonging to the I-S1 sub-group having the amino acid sequence (SEQ ID NO:

• 43):
• 13 1 10 20 30 A-Q-T-V- P-Y-G-I-P-L-I-K-A-D-K-V-Q-A-Q-G--F-K-G-A-N-V-K-V-A-V 40 50 60 L-D-T-G-I-Q-A-S-H-P-D-L-N-V-V-G-G-A-S-F-V-A-G-E-A-*-Y-N-T-D 70 80 90 G-N-G-H-G-T-H-V-A-G-T-V-A-A-L-D-N-T-T-G-V-L-G-V-A-P-S-V-S-L 95a 110 120 Y-A-V-K-V-X-L-N-S-S-G-S-G-T-Y-S-G-I-V-S-G-I-E-W-A-T-T-N-G-M-D 130 140 150 V-I-N-M-S-L-G-G-P-S-G-S-T-A-M-K-Q-A-V-D-N-A-Y-A-R-G-V-V-V-V 160 170 180 A-A-A-G-N-S-G-S-S-G-N-T-N-T-I-G-Y-P-A-K-Y-D-S-V-I-A-V-G-A-V 190 200 210 D-S-N-S-N-R-A-S-F-S-S-V-G-A-E-L-E-V-M-A-P-G-A-G-V-Y-S-T-Y-P 220 230 240 T-S-T-Y-A-T-L-N-G-T-S-M-A-S-P-H-V-A-G-A-A-A-L-I-L-S-K-H-P-N 250 260 270 L-S-A-S-Q-V-R-N-R-L-S-S-T-A-T-Y-L-G-S-S-F-Y-Y-G-K-G-L-I-N-V 275 E-A-A-A-Q
• or a homologous subtilase having an amino acid sequence comprising a position 95a amino acid residue and exhibiting an identity of more than 70%, 75%, 80%, 85%, 90%, or 95% therewith.

A subtilase belonging to the I-S2 sub-group having the amino acid sequence (SEQ ID NO:

• 44):
• 14 1 10 20 30 A-Q-S-V-P-W-G-I-S-R-V-Q-A-P-A-A-H-N-R-G-L-T-G-S-G-V-K-V-A-V- 40 50 60 L-D-T-G-I-*-S-T-H-P-D-L-N-I-R-G-G-A-S-F-V-P-G-E-P-*-S-T-Q-D- 70 80 90 G-N-G-H-G-T-H-V-A-G-T-I-A-A-L-N-N-S-I-G-V-L-G-V-A-P-S-A-E-L- 95a 110 120 Y-A-V-K-V-X-L-G-A-S-G-S-G-S-V-S-S-I-A-Q-G-L-E-W-A-G-N-N-G-M-H- 130 140 150 V-A-N-L-S-L-G-S-P-S-P-S-A-T-L-E-Q-A-V-N-S-A-T-S-R-G-V-L-V-V- 160 170 180 A-A-S-G-N-S-G-A-*-G-S-I-S-*-*-*-Y-P-A-R-Y-A-N-A-M-A-V-G-A-T- 190 200 210 D-Q-N-N-N-R-A-S-F-S-Q-Y-G-A-G-L-D-I-V-A-P-G-V-N-V-Q-S-T-Y-P- 220 230 240 G-S-T-Y-A-S-L-N-G-T-S-M-A-T-P-H-V-A-G-A-A-A-L-V-K-Q-K-N-P-S- 250 260 270 W-S-N-V-Q-I-R-N-H-L-K-N-T-A-T-S-L-G-S-T-N-L-Y-G-S-G-L-V-N-A- 275 E-A-A-T-R
• or a homologous subtilase having an amino acid sequence comprising a position 95a amino acid residue and exhibiting an identity of more than 70%, 75%, 80%, 85%, 90%, or 95% therewith.

A subtilase belonging to the I-S1 sub-group having the amino acid sequence (SEQ ID NO:

• 45):
• 15 1 10 20 30 A-Q-T-V-P-Y-G-I-P-L-I-K-A-D-K-V-Q-A-Q-G-F-K-G-A-N-V-K-V-A-V 40 50 60 L-D-T-G-I-Q-A-S-H-P-D-L-N-V-V-G-G-A-S-F-V-A-G-E-A-*-Y-N-T-D 70 80 90 G-N-G-H-G-T-H-V-A-G-T-V-A-A-L-D-N-T-T-G-V-L-G-V-A-P-S-V-S-L 96a 110 120 Y-A-V-K-V-L-X-N-S-S-G-S-G-T-Y-S-G-I-V-S-G-I-E-W-A-T-T-N-G-M-D 130 140 150 V-I-N-M-S-L-G-G-P-S-G-S-T-A-M-K-Q-A-V-D-N-A-Y-A-R-G-V-V-V-V 160 170 180 A-A-A-G-N-S-G-S-S-G-N-T-N-T-I-G-Y-P-A-K-Y-D-S-V-I-A-V-G-A-V 190 200 210 D-S-N-S-N-R-A-S-F-S-S-V-G-A-E-L-E-V-M-A-P-G-A-G-V-Y-S-T-Y-P 220 230 240 T-S-T-Y-A-T-L-N-G-T-S-M-A-S-P-H-V-A-G-A-A-A-L-I-L-S-K-H-P-N 250 260 270 L-S-A-S-Q-V-R-N-R-L-S-S-T-A-T-Y-L-G-S-S-F-Y-Y-G-K-G-L-I-N-V 275 E-A-A-A-Q
• or a homologous subtilase having an amino acid sequence comprising a position 96a amino acid residue and exhibiting an identity of more than 70%, 75%, 80%, 85%, 90%, or 95% therewith.

A subtilase belonging to the I-S2 sub-group having the amino acid sequence (SEQ ID NO:

• 46):
• 16 1 10 20 30 A-Q-S-V-P-W-G-I-S-R-V-Q-A-P-A-A-H-N-R-G-L-T-G-S-G-V-K-V-A-V- 40 50 60 L-D-T-G-I-*-S-T-H-P-D-L-N-I-R-G-G-A-S-F-V-P-G-E-P-*-S-T-Q-D- 70 80 90 G-N-G-H-G-T-H-V-A-G-T-I-A-A-L-N-N-S-I-G-V-L-G-V-A-P-S-A-E-L- 96a 110 120 Y-A-V-K-V-L-X-G-A-S-G-S-G-S-V-S-S-I-A-Q-G-L-E-W-A-G-N-N-G-M-H- 130 140 150 V-A-N-L-S-L-G-S-P-S-P-S-A-T-L-E-Q-A-V-N-S-A-T--S-R-G-V-L-V-V- 160 170 180 A-A-S-G-N-S-G-A-*-G-S-I-S-*-*-*-Y-P-A-R-Y-A-N-A-M-A-V-G-A-T- 190 200 210 D-Q-N-N-N-R-A-S-F-S-Q-Y-G-A-G-L-D-I-V-A-P-G-V-N-V-Q-S-T-Y-P- 220 230 240 G-S-T-Y-A-S-L-N-G-T-S-M-A-T-P-H-V-A-G-A-A-A-L-V-K-Q-K-N-P-S- 250 260 270 W-S-N-V-Q-I-R-N-H-L-K-N-T-A-T-S-L-G-S-T-N-L-Y-G-S-0-L-V-N-A- 275 E-A-A-T-R
• or a homologous subtilase having an amino acid sequence comprising a position 96a amino acid residue and exhibiting an identity of more than 70%, 75%, 80%, 85%, 90%, or 95% therewith.

A subtilase belonging to the I-S1 sub-group having the amino acid sequence (SEQ ID NO:

• 47):
• 17 1 10 20 30 A-Q-T-V-P-Y-G-I-P-I-I-K-A-D-K-V-Q-A-Q-G-F-K-G-A-N-V-K-V-A-V 40 50 60 L-D-T-G-I-Q-A-S-H-P-D-L-N-V-V-G-G-A-S-F-V-A-G-E-A-*-Y-N-T-D 70 80 90 G-N-G-H-G-T-H-V-A-G-T-V-A-A-L-D-N-T-T-G-V-L-G-W-A-P-S-V-S-L 97a 100 110 120 Y-A-V-K-V-L-N-X-S-S-G-S-G-T-Y-S-G-I-V-S-G-I-E-W-A-T-T-N-G-M-D 130 140 150 V-I-N-M-S-L-G-G-P-S-G-S-T-A-M-K-Q-A-V-D-N-A-Y-A-R-G-V-V-V-V 160 170 180 A-A-A-G-N-S-G-S-S-G-N-T-N-T-I-G-Y-P-A-K-Y-D-S-V-I-A-V-G-A-V 190 200 210 D-S-N-S-N-R-A-S-F-S-S-V-G-A-E-L-E-V-M-A-P-G-A-G-V-Y-S-T-Y-P 220 230 240 T-S-T-Y-A-T-L-N-G-T-S-M-A-S-P-H-V-A-G-A-A-A-L-I-L-S-K-H-P-N 250 260 270 L-S-A-S-Q-V-R-N-R-L-S-S-T-A-T-Y-L-G-S-S-F-Y-Y-G-K-G-L-I-N-V 275 E-A-A-A-Q
• or a homologous subtilase having an amino acid sequence comprising a position 97a amino acid residue and exhibiting an identity of more than 70%, 75%, 80%, 85%, 90%, or 95% therewith.

A subtilase belonging to the I-S2 sub-group having the amino acid sequence (SEQ ID NO:

• 48):
• 18 1 10 20 30 A-Q-S-V-P-W-G-I-S-R-V-Q-A-P-A-A-H-N-R-G-L-T-G-S-G-V-K-V-A-V- 40 50 60 L-D-T-G-I-*-S-T-H-P-D-L-N-I-R-G-G-A-S-F-V-P-G-E-P-*-S-T-Q-D- 70 80 90 G-N-G-H-G-T-H-V-A-G-T-I-A-A-L-N-N-S-I-G-V-L-G-V-A-P-S-A-E-L- 97a 100 110 120 Y-A-V-K-V-L-G-X-A-S-G-S-G-S-V-S-S-I-A-Q-G-L-E-W-A-G-N-N-G-M-H- 130 140 150 V-A-N-L-S-L-G-S-P-S-P-S-A-T-L-E-Q-A-V-N-S-A-T-S-R-G-V-L-V-V- 160 170 180 A-A-S-G-N-S-G-A-*-G-S-I-S-*-*-*-Y-P-A-R-Y-A-N-A-M-A-V-G-A-T- 190 200 210 D-Q-N-N-N-R-A-S-F-S-Q-Y-G-A-G-L-D-I-V-A-P-G-V-N-V-Q-S-T-Y-P- 220 230 240 G-S-T-Y-A-S-L-N-G-T-S-M-A-T-P-H-V-A-G-A-A-A-L-V-K-Q-K-N-P-S- 250 260 270 W-S-N-V-Q-I-R-N-H-L-K-N-T-A-T-S-L-G-S-T-N-L-Y-G-S-G-L-V-N-A- 275 E-A-A-T-R
• or a homologous subtilase having an amino acid sequence compris-ing a position 97a amino acid residue and exhibiting an identity of more than 70%, 75%, 80%, 85%, 90%, or 95% therewith.

A subtilase belonging to the I-S1 sub-group having the amino acid sequence (SEQ ID NO:

• 49):
• 19 1 10 20 30 A-Q-T-V-P-Y-G-I-P-L-I-K-A-D-K-V-Q-A-Q-G-F-K-G-A-N-V-K-V-A-V 40 50 60 L-D-T-G-I-Q-A-S-H-P-D-L-N-V-V-G-G-A-S-F-V-A-G-E-A-*-Y-N-T-D 70 80 90 G-N-G-H-G-T-H-V-A-G-T-V-A-A-L-D-N-T-T-G-V-L-G-V-A-P-S-V-S-L 98a 110 120 Y-A-V-K-V-L-N-S-X-S-G-S-G-T-Y-S-G-I-V-S-G-T-E-W-A-T-T-N-G-M-D 130 140 150 V-I-N-M-S-L-G-G-P-S-G-S-T-A-M-K-Q-A-V-D-N-A-Y-A-R-G-V-V-V-V 160 170 180 A-A-A-G-N-S-G-S-S-G-N-T-N-T-I-G-Y-P-A-K-Y-D-S-V-I-A-V-G-A-V 190 200 210 D-S-N-S-N-R-A-S-F-S-S-V-G-A-E-L-E-V-M-A-P-G-A-G-V-Y-S-T-Y-P 220 230 240 T-S-T-Y-A-T-L-N-G-T-S-M-A-S-P-H-V-A-G-A-A-A-L-I-L-S-K-H-P-N 250 260 1270 L-S-A-S-Q-V-R-N-R-L-S-S-T-A-T-Y-L-G-S-S-F-Y-Y-G-K-G-L-I-N-V 275 E-A-A-A-Q
• or a homologous subtilase having an amino acid sequence comprising a position 98a amino acid residue and exhibiting an identity of more than 70%, 75%, 80%, 85%, 90%, or 95% therewith.

A subtilase belonging to the I-S2 sub-group having the amino acid sequence (SEQ ID NO:

• 50):
• 20 1 10 20 30 A-Q-S-V-P-W-G-I-S-R-V-Q-A-P-A-A-H-N-R-G-L-T-G-S-G-V-K-V-A-V 40 50 60 L-D-T-G-I-*-S-T-H-P-D-L-N-I-R-G-G-A-S-F-V-P-G-E-P-*-S-T-Q-D 70 80 90 G-N-G-H-G-T-H-V-A-G-T-I-A-A-L-N-N-S-I-G-V-L-G-V-A-P-S-A-E-L 98a 110 120 Y-A-V-K-V-L-G-A-X-S-G-S-G-S-G-S-S-I-A-Q-G-L-E-W-A-G-N-N-G-M-H 130 140 150 V-A-N-L-S-L-G-S-P-S-P-S-A-T-L-E-Q-A-V-N-S-A-T-S-R-G-V-L-V-V 160 170 180 A-A-S-G-N-S-G-A-*-G-S-I-S-*-*-*-Y-P-A-R-Y-A-N-A-M-A-V-G-A-T 190 200 210 D-Q-N-N-N-R-A-S-F-S-Q-Y-G-A-G-L-D-I-V-A-P-G-V-N-V-Q-S-T-Y-P 220 230 240 G-S-T-Y-A-S-L-N-G-T-S-M-A-T-P-H-V-A-G-A-A-A-L-V-K-Q-K-N-P-S 250 260 270 W-S-N-V-Q-I-R-N-H-L-K-N-T-A-T-S-L-G-S-T-N-L-Y-G-S-G-L-V-N-A- 275 E-A-A-T-R
• or a homologous subtilase having an amino acid sequence comprising a position 98a amino acid residue and exhibiting an identity of more than 70%, 75%, 80%, 85%, 90%, or 95% therewith.

A subtilase belonging to the I-S1 sub-group having the amino acid is sequence (SEQ ID NO:

• 51):
• 21 1 10 20 130 A-Q-T-V-P-Y-G-I-P-L-I-K-A-D-K-V-Q-A-Q-G-F-K-G-A-N-V-K-V-A-V 40 50 60 L-D-T-G-I-Q-A-S-H-P-D-L-N-V-V-G-G-A-S-F-V-A-G-E-A-*-Y-N-T-D 70 80 90 G-N-G-H-G-T-H-V-A-G-T-V-A-A-L-D-N-T-T-G-V-L-Q-V-A-P-S-V-S-L 99a 110 120 Y-A-V-K-V-L-N-S-S-X-G-S-G-T-Y-S-G-I-V-S-G-I-E-W-A-T-T-N-G-M-D 130 140 150 V-I-N-M-S-L-G-G-P-S-G-S-T-A-M-K-Q-A-V-D-N-A-Y-A-R-G-V-V-V-V 160 170 180 A-A-A-G-N-S-G-S-S-G-N-T-N-T-I-G-Y-P-A-K-Y-D-S-V-I-A-V-G-A-V 190 200 210 D-S-N-S-N-R-A-S-F-S-S-V-G-A-E-L-E-V-M-A-P-G-A-G-V-Y-S-T-Y-P 220 230 240 T-S-T-Y-A-T-L-N-G-T-S-M-A-S-P-H-V-A-G-A-A-A-L-I-L-S-K-H-P-N 250 260 270 L-S-A-S-Q-V-R-N-R-L-S-S-T-A-T-Y-L-G-S-S-F-Y-Y-G-K-G-L-I-N-V 275 E-A-A-A-Q
• or a homologous subtilase having an amino acid sequence comprising a position 99a amino acid residue and exhibiting an identity of more than 70%, 75%, 80%, 85%, 90%, or 95% therewith.

A subtilase belonging to the I-S2 sub-group having the amino acid sequence (SEQ ID NO:

• 52):
• 22 1 10 20 30 A-Q-S-V-P-W-G-I-S-R-V-Q-A-P-A-A-H-N-R-G-L-T-G-S-G-V-K-V-A-V 40 50 60 L-D-T-G-I-*-S-T-R-P-D-L-N-I-R-G-G-A-S-F-V-P-G-E-P-*-S-T-Q-D 70 80 90 G-N-G-H-G-T-H-V-A-G-T-I-A-A-L-N-N-S-I-G-V-L-G-V-A-P-S-A-E-L 99a 110 120 Y-A-V-K-V-L-G-A-S-X-G-S-G-S-G-S-S-I-A-Q-G-L-E-W-A-G-N-N-G-M-H 130 140 150 V-A-N-L-S-L-G-S-P-S-P-S-A-T-L-E-Q-A-V-N-S-A-T-S-R-G-V-L-V-V 160 170 180 A-A-S-G-N-S-G-A-*-G-S-I-S-*-*-*-Y-P-A-R-Y-A-N-A-M-A-V-G-A-T 190 200 210 D-Q-N-N-N-R-A-S-F-S-Q-Y-G-A-G-L-D-I-V-A-P-G-V-N-V-Q-S-T-Y-P 220 230 240 G-S-T-Y-A-S-L-N-G-T-S-M-A-T-P-H-V-A-G-A-A-A-L-V-K-Q-K-N-P-S- 250 260 270 W-S-N-V-Q-I-R-N-H-L-K-N-T-A-T-S-L-G-S-T-N-L-Y-G-S-G-L-V-N-A- 275 E-A-A-T-R
• or a homologous subtilase having an amino acid sequence comprising a position 99a amino acid residue and exhibiting an identity of more than 70%, 75%, 80%, 85%, 90%, or 95% therewith.

A subtilase belonging to the I-S1 sub-group having the amino acid sequence (SEQ ID NO:

• 53):
• 23 1 10 20 30 A-Q-T-V-P-Y-G-I-P-L-I-K-A-D-K-V-Q-A-Q-G-F-K-G-A-N-V-K-V-A-V 40 50 60 L-D-T-G-I-Q-A-S-H-P-D-L-N-V-V-G-G-A-S-F-V-A-G-E-A-*-Y-N-T-D 70 80 90 G-N-G-H-G-T-H-V-A-G-T-V-A-A-L-D-N-T-T-G-V-L-G-V-A-P-S-V-S-L 100a 110 120 Y-A-V-K-V-L-N-S-S-G-X-S-G-T-Y-S-G-I-V-S-G-I-E-W-A-T-T-N-G-M-D 130 140 150 V-I-N-M-S-L-G-G-P-S-G-S-T-A-M-K-Q-A-V-D-N-A-Y-A-R-G-V-V-V-V 160 170 180 A-A-A-G-N-S-G-S-S-G-N-T-N-T-I-G-Y-P-A-K-Y-D-S-V-I-A-V-G-A-V 190 200 210 D-S-N-S-N-R-A-S-F-S-S-V-G-A-E-L-E-V-M-A-P-G-A-G-V-Y-S-T-Y-P 220 230 240 T-S-T-Y-A-T-L-N-G-T-S-M-A-S-P-H-V-A-G-A-A-A-L-I-L-S-K-H-P-N 250 260 270 L-S-A-S-Q-V-R-N-R-L-S-S-T-A-T-Y-L-G-S-S-F-Y-Y-G-K-G-L-I-N-V 275 E-A-A-A-Q
• or a homologous subtilase having an amino acid sequence compris-ing a position 100a amino acid residue and exhibiting an iden-tity of more than 70%, 75%, 80%, 85%, 90%, or 95% therewith.

A subtilase belonging to the I-S2 sub-group having the amino acid sequence (SEQ ID NO:

• 54):
• 24 1 10 20 30 A-Q-S-V-P-W-G-I-S-R-V-Q-A-P-A-A-H-N-R-G-L-T-G-S-G-V-K-V-A-V 40 50 60 L-D-T-G-I-*-S-T-H-P-D-L-N-I-R-G-G-A-S-F-V-P-G-E-P-*-S-T-Q-D 70 80 90 G-N-G-H-G-T-H-V-A-G-T-I-A-A-L-N-N-S-I-G-V-L-G-V-A-P-S-A-E-L 100a 110 120 Y-A-V-K-V-L-G-A-S-G-X-S-G-S-V-S-S-I-A-Q-G-L-E-W-A-G-N-N-G-M-H 130 140 150 V-A-N-L-S-L-G-S-P-S-P-S-A-T-L-E-Q-A-V-N-S-A-T-S-R-G-V-L-V-V 160 170 180 A-A-S-G-N-S-G-A-*-G-S-I-S-*-*-*-Y-P-A-R-Y-A-N-A-M-A-V-G-A-T 190 200 210 D-Q-N-N-N-R-A-S-F-S-Q-Y-G-A-G-L-D-I-V-A-P-G-V-N-V-Q-S-T-Y-P 220 230 240 G-S-T-Y-A-S-L-N-G-T-S-M-A-T-P-H-V-A-G-A-A-A-L-V-K-Q-K-N-P-S- 250 260 270 W-S-N-V-Q-I-R-N-H-L-K-N-T-A-T-S-L-G-S-T-N-L-Y-G-S-G-L-V-N-A- 275 E-A-A-T-R
• or a homologous subtilase having an amino acid sequence comprising a position 100a amino acid residue and exhibiting an identity of more than 70%, 75%, 80%, 85%, 90%, or 95% therewith.

A subtilase belonging to the I-S1sub-group having the amino acid sequence (SEQ ID NO:

• 55):
• 25 1 /10 20 30 A-Q-T-V-P-Y-G-I-P-L-I-K-A-D-K-V-Q-A-Q-G-F-K-G-A-N-V-K-V-A-V 40 50 60 L-D-T-G-I-Q-A-S-H-P-D-L-N-V-V-G-G-A-S-F-V-A-G-E-A-*-Y-N-T-D 70 80 90 G-N-G-H-G-T-H-V-A-G-T-V-A-A-L-D-N-T-T-G-V-L-G-V-A-P-S-V-S-L 101a 110 120 Y-A-V-K-V-L-N-S-S-G-S-X-G-T-Y-S-G-I-V-S-G-I-E-W-A-T-T-N-G-M-D 130 140 150 V-I-N-M-S-L-G-G-P-S-G-S-T-A-M-K-Q-A-V-D-N-A-Y-A-R-G-V-V-V-V 160 170 180 A-A-A-G-N-S-G-S-S-G-N-T-N-T-I-G-Y-P-A-K-Y-D-S-V-I-A-V-G-A-V 190 200 210 D-S-N-S-N-R-A-S-F-S-S-V-G-A-E-L-E-V-M-A-P-G-A-G-V-Y-S-T-Y-P 220 230 240 T-S-T-Y-A-T-L-N-G-T-S-M-A-S-P-H-V-A-G-A-A-A-L-I-L-S-K-H-P-N 250 260 270 L-S-A-S-Q-V-R-N-R-L-S-S-T-A-T-Y-L-G-S-S-F-Y-Y-G-K-G-L-I-N-V 275 E-A-A-A-Q
• or a homologous subtilase having an amino acid sequence comprising a position 101a amino acid residue and exhibiting an iden-tity of more than 70%, 75%, 80%, 85%, 90%, or 95% therewith.

A subtilase belonging to the I-S2 sub-group having the amino acid sequence (SEQ ID NO:

• 56):
• 26 1 10 20 30 A-Q-S-V-P-W-G-I-S-R-V-Q-A-P-A-A-H-N-R-G-L-T-G-S-G-V-K-V-A-V 40 50 60 L-D-T-G-I*-S-T-H-P-D-L-N-I-R-G-G-A-S-F-V-P-G-E-P-*-S-T-Q-D 70 80 90 G-N-G-H-G-T-H-V-A-G-T-I-A-A-L-N-N-S-I-G-V-L-G-V-A-P-S-A-E-L 101a 110 120 Y-A-V-K-V-L-G-A-S-G-S-X-G-S-V-S-S-I-A-Q-G-L-E-W-A-G-N-N-G-M-H 130 140 150 V-A-U-L-S-L-G-S-P-S-P-S-A-T-L-E-Q-A-V-N-S-A-T-S-R-G-V-L-V-V 160 170 180 A-A-S-G-N-S-G-A-*-G-S-I-S-*-*-*-Y-P-A-R-Y-A-N-A-M-A-V-G-A-T 190 200 210 D-Q-N-N-N-R-A-S-F-S-Q-Y-G-A-G-L-D-I-V-A-P-G-V-N-V-Q-S-T-Y-P 220 230 240 G-S-T-Y-A-S-L-N-G-T-S-M-A-T-P-H-V-A-G-A-A-A-L-V-K-Q-K-N-P-S- 250 260 270 W-S-N-V-Q-I-R-N-H-L-K-N-T-A-T-S-L-G-S-T-N-L-Y-G-S-G-L-V-N-A- 275 E-A-A-T-R
• or a homologous subtilase having an amino acid sequence comprising a position 101a amino acid residue and exhibiting an identity of more than 70%, 75%, 80%, 85%, 90%, or 95% therewith.

A subtilase belonging to the I-S1 sub-group having the amino acid sequence (SEQ ID NO:

• 57):
• 27 1 10 20 30 A-Q-T-V-P-Y-G-I-P-L-I-K-A-D-K-V-Q-A-Q-G-F-K-G-A-N-V-K-V-A-V 40 50 60 L-D-T-G-I-Q-A-S-H-P-D-L-N-V-V-G-G-A-S-F-V-A-G-E-A-*-Y-N-T-D 70 80 90 G-N-G-H-G-T-H-V-A-G-T-V-A-A-L-D-N-T-T-G-V-L-G-V-A-P-S-V-S-L 102a 110 120 Y-A-V-K-V-L-N-S-S-G-S-G-X-T-Y-S-G-I-V-S-G-I-E-W-A-T-T-N-G-M-D 130 140 150 V-I-N-M-S-L-G-G-P-S-G-S-T-A-M-K-Q-A-V-D-N-A-Y-A-R-G-V-V-V-V 160 170 180 A-A-A-G-N-S-G-S-S-G-N-T-N-T-I-G-Y-P-A-K-Y-D-S-V-I-A-V-G-A-V 190 200 210 D-S-N-S-N-R-A-S-F-S-S-V-G-A-E-L-E-V-M-A-P-G-A-G-V-Y-S-T-Y-P 220 230 240 T-S-T-Y-A-T-L-N-G-T-S-M-A-S-P-H-V-A-G-A-A-A-L-I-L-S-K-H-P-N 250 260 270 L-S-A-S-Q-V-R-N-R-L-S-S-T-A-T-Y-L-G-S-S-F-Y-Y-G-K-G-L-I-N-V 275 E-A-A-A-Q
• or a homologous subtilase having an amino acid sequence comprising a position 102a amino acid residue and exhibiting an identity of more than 70%, 75%, 80%, 85%, 90%, or 95% therewith.

A subtilase belonging to the I-S2 sub-group having the amino acid sequence (SEQ ID NO:

• 58):
• 28 1 10 20 30 A-Q-S-V-P-W-G-I-S-R-V-Q-A-P-A-A-H-N-R-G-L-T-G-S-G-V-K-V-A-V 40 50 60 L-D-T-G-I-*-S-T-R-P-D-L-N-I-R-G-G-A-S-F-V-P-G-E-P-*-S-T-Q-D 70 80 90 G-N-G-H-G-T-H-V-A-G-T-I-A-A-L-N-N-S-I-G-V-L-G-V-A-P-S-A-E-L 102a 110 120 Y-A-V-K-V-L-G-A-S-G-S-G-X-S-V-S-S-I-A-Q-G-L-E-W-A-G-N-N-G-M-H 130 140 150 V-A-N-L-S-L-G-S-P-S-P-S-A-T-L-E-Q-A-V-N-S-A-T-S-R-G-V-L-V-V 160 170 180 A-A-S-G-N-S-G-A-*-G-S-I-S-*-*-*-Y-P-A-R-Y-A-N-A-M-A-V-G-A-T 190 200 210 D-Q-N-N-N-R-A-S-F-S-Q-Y-G-A-G-L-D-I-V-A-P-G-V-N-V-Q-S-T-Y-P 220 230 240 G-S-T-Y-A-S-L-N-G-T-S-M-A-T-P-H-V-A-G-A-A-A-L-V-K-Q-K-N-P-S- 250 260 270 W-S -N-V-Q-I-R-N-H-L-K-N-T-A-T-S-L-G-S-T-N-L-Y-G-S-G-L-V-N-A- 275 E-A-A-T-R
• or a homologous subtilase having an amino acid sequence comprising a position 102a amino acid residue and exhibiting an identity of more than 70%, 75%, 80%, 85%, 90%, or 95% therewith.

A subtilase belonging to the I-S1 sub-group having the amino acid sequence (SEQ ID NO:

• 59):
• 29 1 10 20 30 A-Q-T-V-P-Y-G-I-P-L-I-K-A-D-K-V-Q-A-Q-G-F-K-G-A-N-V-K-V-A-V 40 50 60 L-D-T-G-I-Q-A-S-H-P-D-L-N-V-V-G-G-A-S-F-V-A-G-E-A-*-Y-N-T-D 70 80 90 G-N-G-H-G-T-H-V-A-G-T-V-A-A-L-D-N-T-T-G-V-L-G-V-A-P-S-V-S-L 103a 110 120 Y-A-V-K-V-L-N-S-S-G-S-G-T-X-Y-S-G-I-V-S-G-I-E-W-A-T-T-N-G-M-D 130 140 150 V-I-N-M-S-L-G-G-P-S-G-S-T-A-M-K-Q-A-V-D-N-A-Y-A-R-G-V-V-V-V 160 170 180 A-A-A-G-N-S-G-S-S-G-N-T-N-T-I-G-Y-P-A-K-Y-D-S-V-I-A-V-G-A-V 190 200 210 D-S-N-S-N-R-A-S-F-S-S-V-G-A-E-L-E-V-M-A-P-G-A-G-V-Y-S-T-Y-P 220 230 240 T-S-T-Y-A-T-L-N-G-T-S-M-A-S-P-H-V-A-G-A-A-A-L-I-L-S-K-H-P-N 250 260 270 L-S-A-S-Q-V-R-N-R-L-S-S-T-A-T-Y-L-G-S-S-F-Y-Y-G-K-G-L-I-N-V 275 E-A-A-A-Q
• or a homologous subtilase having an amino acid sequence comprising a position 103a amino acid residue and exhibiting an identity of more is than 70%, 75%, 80%, 85%, 90%, or 95% therewith.

A subtilase belonging to the I-S2 sub-group having the amino acid sequence (SEQ ID NO:

• 60):
• 30 1 10 20 30 A-Q-S-V-P-W-G-I-S-R-V-Q-A-P-A-A-H-N-R-G-L-T-G-S-G-V-K-V-A-V- 40 50 60 L-D-T-G-I-*-S-T-H-P-D-L-N-I-R-G-G-A-S-F-V-P-G-E-P-*-S-T-Q-D- 70 80 90 G-N-G-H-G-T-H-V-A-G-T-I-A-A-L-N-N-S-I-G-V-L-G-V-A-P-S-A-E-L- 103a 110 120 Y-A-V-K-V-L-G-A-S-G-S-G-S-X-V-S-S-I-A-Q-G-L-E-W-A-G-N-N-G-M-H- 130 140 150 V-A-N-L-S-L-G-S-P-S-P-S-A-T-L-E-Q-A-V-N-S-A-T-S-R-G-V-L-V-V- 160 170 180 A-A-S-G-N-S-G-A-*-G-S-I-S-*-*-*-Y-P-A-R-Y-A-N-A-M-A-V-G-A-T- 190 200 210 D-Q-N-N-N-R-A-S-F-S-Q-Y-G-A-G-L-D-I-V-A-P-G-V-N-V-Q-S-T-Y-P- 220 230 240 G-S-T-Y-A-S-L-N-G-T-S-M-A-T-P-H-V-A-G-A-A-A-L-V-K-Q-K-N-P-S- 250 260 270 W-S-N-V-Q-I-R-N-H-L-K-N-T-A-T-S-L-G-S-T-N-L-Y-G-S-G-L-V-N-A- 275 E-A-A-T-R
• or a homologous subtilase having an amino acid sequence comprising a position 103a amino acid residue and exhibiting an identity of more than 70%, 75%, 80%, 85%, 90%, or 95% therewith.