Compounds, antibodies, reagent kits, methods of producing antibodies, and methods of detecting analytes
AssigneeRoche Diagnostics GmbH
InventorsRaymond A. Hui, Richard T. Root, Stephen S. Vitone
Life of this patent
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Abstract
Compounds including haptens, intermediates, and immunogens that are useful in the production of antibodies specific for the methylenedioxy class of amphetamine derivatives are described. Antibodies specific for the methylenedioxy class of amphetamine derivatives, reagent kits containing antibodies specific for the methylenedioxy class of amphetamine derivatives, methods of producing antibodies specific for the methylenedioxy class of amphetamine derivatives, and methods of detecting analytes including members of the methylenedioxy class of amphetamine derivatives are also described.
Claims45
A compound having a structure
- 9
- wherein:
- R1 is an alkyl group comprising 2-6 carbon atoms;
- R2 is selected from the group consisting of hydrogen, an alkyl group, and a protecting group;
- R3 is an optionally substituted alkyl group;
- and
- Z is -L-X-Q;
- wherein
- L comprises 1-15 carbon atoms and 0-6 heteroatoms;
- X is selected from the group consisting of —O—, —CO—, —NR4—, —S—, —C(═NH)O—, —NH(CO)—, —NH(CO)NH—, —NH(CS)—, —NH(CS)NH—, —O(CO)NH—, —NH(C═NH)—, and maleimidothioether, wherein R4 is selected from the group consisting of hydrogen and an alkyl group;
- and
- Q is selected from the group consisting of hydrogen, a hydroxyl, a leaving group, a macromolecular carrier, and a label:
An antibody specific for MDEA.
An antibody specific for an analyte wherein the analyte comprises a structure
- 10
- wherein:
- R1 is an alkyl group comprising 2-6 carbon atoms;
- R2 is selected from the group consisting of hydrogen, an alkyl group, and a protecting group;
- R3 is an optionally substituted alkyl group;
- and
- Z is -L-X-Q;
- wherein
- L comprises 1-15 carbon atoms and 0-6 heteroatoms;
- X is selected from the group consisting of —O—, —CO—, —NR4—, —S—, —C(═NH)O—, —NH(CO)—, —NH(CO)NH—, —NH(CS)—, —NH(CS)NH—, —O(CO)NH—, —NH(C═NH)—, and maleimidothioether, wherein R4 is selected from the group consisting of hydrogen and an alkyl group;
- and
- Q is selected from the group consisting of hydrogen, a hydroxyl, a leaving group, a macromolecular carrier, and a label.
A method of producing an antibody comprising inoculating a host with an immunogen comprising a structure
- 11
- wherein:
- R1 is an alkyl group comprising 2-6 carbon atoms;
- R2 is selected from the group consisting of hydrogen, an alkyl group, and a protecting group;
- R3 is an optionally substituted alkyl group;
- and
- Z is -L-X-Q;
- wherein
- L comprises 1-15 carbon atoms and 0-6 heteroatoms;
- X is selected from the group consisting of —O—, —CO—, —N R4—, —S—, —C(═NH)O—, —NH(CO)—, —NH(CO)NH—, —NH(CS)—, —NH(CS)NH—, —O(CO)NH—, —NH(C═NH)—, and maleimidothioether, wherein R4 is selected from the group consisting of hydrogen and an alkyl group;
- and
- Q is a macromolecular carrier.
Dependencies are inferred from the claim text.