Multiplex analysis using membrane-bound sensitizers
AssigneeAclara Biosciences, Inc.
InventorsSharat Singh, Po-Ying Chan-Hui
Life of this patent
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Abstract
The present invention is directed to methods and compositions for determining the presence, absence, and/or amounts of one or more membrane-associated analytes in a sample. In accordance with the invention, binding compounds derivatized with releasable molecular tags specifically bind to selected membrane-associated analytes, after which the molecular tags are released upon activation of cleavage moieties, or sensitizers, anchored in the same membrane as the membrane-associated analytes. The released molecular tags are then identified by their distinct separation and detection characteristics.
Claims20
A method for determining the presence or absence of one or more membrane-associated analytes, the method comprising the steps of:
- providing a sensitizer-treated membrane comprising the one or more membrane-associated analytes;
- providing one or more binding compounds, such that there is at least one binding compound specific for each of the one or more membrane-associated analytes, each binding compound having one or more molecular tags, each molecular tag being attached by a cleavable linkage, and the molecular tags of each binding compound being distinguishable from those of every other binding compound by one or more physical and/or optical characteristics;
- combining the sensitizer-treated membrane and the plurality of binding compounds specific for the one or more membrane-associated analytes such that in the presence of a membrane-associated analyte a complex is formed between such membrane-associated analyte and a binding compound specific therefor;
- activating the sensitizer-treated membrane so that an active species is produced that cleaves the cleavable linkage of the binding compounds forming such complex so that molecular tags are released;
- and
- separating and identifying the released molecular tags by the one or more physical characteristics to determine the one or more membrane-associated analytes in the sample.
A composition for determining the presence or absence of one or more membrane-associated analytes, the composition comprising:
- a sensitizer-treated membrane containing one or more membrane-associated analytes;
- and
- one or more binding compounds, such that there is at least one binding compound specific for each of the one or more membrane-associated analytes, each binding compound having one or more molecular tags, each molecular tag being attached by a cleavable linkage, and the molecular tags of each binding compound being distinguishable from those of every other binding compound by one or more physical and/or optical characteristics;
- wherein at least one binding compound of the plurality is specifically bound to at least one membrane-associated analyte.
A kit for determining the presence or absence of one or more membrane-associated analytes, the kit comprising:
- a lipophilic photosensitizer for generating a photosensitizer-treated membrane having one or more membrane-associated analytes;
- and
- one or more binding compounds, such that there is at least one binding compound specific for each of the one or more membrane-associated analytes, each binding compound having one or more molecular tags, each molecular tag being attached by a cleavable linkage, and the molecular tags of each binding compound being distinguishable from those of every other binding compound by one or more physical and/or optical characteristics.
Dependencies are inferred from the claim text.