Replicable probe array

A method for replicating a specific binding ligand probe array, the method comprising:

• a) providing a master array comprising a support surface having a plurality of address ligands immobilized thereon;
• b) providing a plurality of multi-ligand conjugates, each multi-ligand conjugate comprising a core having attached thereto:
• (i) at least one molecule of a first ligand binding domain, comprising a ligand selected to bind in a specific manner with a particular address ligand of the master array, (ii) at least one molecule of a second ligand binding domain, comprising a ligand selected to bind in a specific manner with a target ligand, and (iii) at least one molecule of a third ligand, selected from the group consisting of binding ligands and polymerizable groups;
• c) attaching the multi-ligand conjugates to the master array by allowing the first ligand binding domains to bind in a specific manner with complementary address ligands;
• d) providing an assay array support comprising a support surface for the replicate array;
• e) bringing the assay array support into sufficient proximity with the master array, under conditions suitable to permit the attached multi-ligand conjugates to attach to the assay array support;
• and
• f) disassociating the bound complementary address ligand and first ligand binding domain under conditions suitable to permit the assay array support to be recovered and used.