Accessory functions for use in recombinant AAV virion production
AssigneeAvigen, Inc.
InventorPeter C. Colosi
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Abstract
Accessory functions capable of supporting efficient recombinant AAV (rAAV) virion production in a suitable host cell are provided. The accessory functions are in the form of one or more vectors that are capable of being transferred between cells. Methods of producing rAAV virions are also provided. The methods can be practiced to produce commercially significant levels of rAAV particles without also generating significant levels of infectious helper virus or other contaminating by-products.
Claims33
A nucleic acid molecule which provides one or more accessory functions for supporting recombinant AAV (rAAV) virion production in a suitable host cell and that lacks at least one adenoviral late gene region, said molecule comprising a nucleotide sequence selected from the group consisting of (i) a sequence that provides adenovirus VA RNAs, (ii) an adenovirus E4 ORF6 coding region, (iii) an adenovirus E2a 72 kD coding region, and any combination of nucleotide sequences (i), (ii) and (iii).
A nucleic acid molecule which provides accessory functions for supporting efficient recombinant AAV (rAAV) virion production in a suitable host cell and that lacks at least one adenoviral late gene region.
An accessory function system for recombinant AAV (rAAV) virion production, comprising first, second, and third accessory function vectors, wherein:
- (a) said first accessory function vector comprises a nucleotide sequence that provides adenovirus VA RNAs;
- (b) said second accessory function vector comprises an adenovirus E4 ORF6 coding region;
- and
- (c) said third accessory function vector comprises an adenovirus region coding for the E2a 72 kD protein.
Dependencies are inferred from the claim text.