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AssigneeOrigen Therapeutics, Inc.
InventorsMarie-Cecile Van de Lavoir, Robert J. Etches, Babette Heyer, Jennifer Diamond, Christine Mather, Kathleen Beemer, Heather Myers
Estimating the term…
Sustained cultures of avian embryonic stem cells are provided. Injection of avian embryonic stem cells into recipient embryos yields chimeras with a significant contribution from the embryonic stem cell phenotype. Transgene encoding exogenous proteins are stably integrated in the embryonic stem cells and are present in the somatic tissue of the resulting chimeras. The transgenes may encode exogenous proteins expressed in endodermal, ectodermal, mesodermal, or extra embryonic tissue. Breeding the resulting chimera yields transgenic birds whose genome is comprised of exogenous DNA.
A composition comprising a sustained culture of chicken embryonic stem cells with a transgene stably integrated into the genome of substantially all of the progeny of the embryonic stem cells and wherein the embryonic stem cell progeny contribute to a somatic tissue of a chimeric chicken produced by the injection of the ES cell progeny into a chicken embryo.
A composition comprising a culture of chicken cells with a transgene is stably integrated in the genome of the cells, wherein the cells express an embryonic stem cell phenotype after being sustained in culture for more than 60 days.
A composition of transgenic chicken embryonic stem cells in culture, wherein embryonic stem cell progeny contain a stably integrated transgene comprised of an operably-linked promoter, a selectable marker, and DNA encoding an exogenous protein, and wherein the embryonic stem cell progeny maintain pluripotency for more than 60 days such that injection into a recipient embryo yields a chimeric chicken expressing the exogenous protein in B lymphocytes of the chimera.
A chimeric chicken comprised of embryonic stem cell progeny having a stably integrated transgene.
A transgenic chicken having a genome comprised of a transgene of greater than 15 kb of exogenous DNA.
A method of creating a chimeric chicken comprising:
A method to produce a chimeric chicken having expression of an exogenous protein in somatic tissue comprising:
Dependencies are inferred from the claim text.