Cellobiohydrolase I gene and improved variants
AssigneeMidwest Research Institute
InventorsWilliam S. Adney, Stephen R. Decker, Suzanne McCarter, John O. Baker, Rafael Nieves, Michael E. Himmel, Todd B. Vinzant
Life of this patent
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Abstract
A nucleic acid molecule having a nucleic acid sequence that encodes a linker region of exoglucanase, said nucleic acid sequence comprising the nucleic sequence 5′-GGCGGAAACCCGCCTGGCACCACC-3′.
Claims18
A nucleic acid molecule having a nucleic acid sequence that encodes a linker region of exoglucanase, said nucleic acid sequence comprising the nucleotide sequence:
- 5′-5 GGCGGAAACCCGCCTGGCACCACC-3′.
9 CCTCCCGGCGGAAACCCGCCTGGCACCACCACCACCCGCCGCCCA-3′
A nucleic acid molecule having a nucleic acid sequence encoding a variant cellobiohydrolase, said nucleic acid sequence comprising a linker region sequence having a length of from about 20 nucleotides to about 50 nucleotides linker region, between a catalytic domain and a cellulose binding domain (CBD).
A method for making an active exoglucanase in a eukaryotic heterologous host, the method comprising reducing glycosylation of the exoglucanase, wherein reducing comprises replacing an N-glycosylation site amino acid residue with non-glycosyl accepting amino acid residue.
An exoglucanase, comprising of the sequence change of Table 4, N45A 5′-GGACTCACGCTACGGCCAGCAGCACGAACTGC-3′.
An exoglucanase, comprising of the sequence change of Table 4, N270A, 5′-CCCATACCGCCTGGGCGCCACCAGCTTCTACGGCCC-3′
An exoglucanase, comprising of the sequence change of Table 4, N384A, 5′-GGACTCCACCTACCCGACAGCCGAGACCTCCTCCACACCCG-3′
Dependencies are inferred from the claim text.