Novel motor proteins and methods for their use
OwnerChristophe Beraud, Richard Freedmaninventors
Life of this patent
Estimating the term…
Abstract
The invention provides isolated nucleic acid and amino acid sequences of HsKif2x, antibodies to HsKif2x, methods of screening for HsKif2x modulators using biologically active HsKif2x, and kits for screening for HsKif2x modulators.
Claims18
An isolated nucleic acid sequence encoding a microtubule motor protein, wherein the motor protein has the following properties:
- (i) the protein's activity includes microtubule stimulated ATPase activity;
- and (ii) the protein has a sequence that has greater than 70% amino acid sequence identity to SEQ ID NO:
- 2 or SEQ ID NO:
- 4 as measured using a sequence comparison algorithm.
An expression vector comprising a nucleic acid encoding a microtubule motor protein, wherein the motor protein has the following properties:
- (i) the protein's activity includes microtubule stimulated ATPase activity;
- and (ii) the protein has a sequence that has greater than 70% amino acid sequence identity to SEQ ID NO:
- 2 or SEQ ID NO:
- 4 as measured using a sequence comparison algorithm.
An isolated microtubule motor protein, wherein the protein has greater than 70% amino acid sequence identity to SEQ ID NO:
- 2 or SEQ ID NO:
- 4 as measured using a sequence comparison algorithm.
A method for screening for modulators of HsKif2x, the method comprising the steps of:
- (i) providing biologically active HsKif2k, wherein has the following properties:
- (i) activity including microtubule stimulated ATPase activity;
- and (ii) sequence that has greater than 70% amino acid sequence identity to HsKif2x of SEQ ID NO:
- 2 or SEQ ID NO:
- 4 as measured using a sequence comparison algorithm;
- (ii) contacting biologically active HsKif2x with a candidate agent in a test and control concentration;
- and
- (iii) assaying for the level of HsKif2x activity, wherein the HsKif2x activity is selected from the group consisting of binding activity or ATPase activity, and wherein a change in activity between the test and control concentration indicates a modulator.
An isolated nucleic acid comprising a sequence which has greater than 60% sequence identity with nucleotide SEQ ID NO:
- 1 or SEQ ID NO:
- 3.
Dependencies are inferred from the claim text.