Recombinant human creatine kinase heterodimer with solution-stability
AssigneeOriental Yeast Co., Ltd.
InventorsKohji Uchida, Hirokazu Matsukawa, Seiichi Kohda, Osamu Oka, Yuhsi Matuo
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Abstract
A recombinant human creatine kinase heterodimer with solution-stability is disclosed. The CK heterodimer is characterized by being produced by using a vector which contains a nucleic acid encoding the M type subunit of creatine kinase and a nucleic acid encoding the B type subunit thereof.
Claims11
A vector comprising a nucleic acid encoding the M type subunit of creatine kinase and a nucleic acid encoding the B type subunit thereof, wherein
- i) said M type subunit is a polypeptide having the amino acid residues 1 to 381 in SEQ ID NO:1, or a polypeptide having an amino acid sequence derived from said sequence by deletion, substitution or addition of one or more amino acid residues and having enzymatic activity;
- and
- ii) said B type subunit is a polypeptide having the amino acid residues 1 to 381 in SEQ ID NO:3, or a polypeptide having an amino acid sequence derived from said sequence by deletion, substitution or addition of one or more amino acid residues and having enzymatic activity.
A solution-stable composition comprising a creatine kinase heterodimer recombinant protein containing the M type subunit and the B type subunit of creatine kinase.
Dependencies are inferred from the claim text.