N-methyltransferase
OwnerBala Rathinasabapathiinventor
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Abstract
A β-alanine N-methyltransferase was isolated from L. latifolium. The purified enzyme catalyzes the N-methylation of β-ala betaine, has an isoelectric point of about 5.15 and an apparent molecular weight of about 43 kilodaltons. The purified enzyme was partially sequenced. The purified enzyme or portions thereof can be used to make antibodies that specifically bind the enzyme, and can be introduced into a cell to modulate the cell's N-methyltransferase activity level and ability to resist environmental stress.
Claims24
A purified N-methyltransferase, the N-methyltransferase:
- (a) being present in Limonium latifolium;
- (b) having an isoelectric point of about 5.15;
- and
- (c) migrating on SDS-PAGE at about 43 kilodaltons.
A purified protein comprising the amino acid sequence of SEQ ID NO:
- 1.
A purified protein comprising the amino acid sequence of SEQ ID NO:
- 2.
A purified protein comprising the amino acid sequence of SEQ ID NO:
- 3
A purified protein comprising the amino acid sequence of SEQ ID NO:
- 4.
A purified protein comprising the amino acid sequence of SEQ ID NO:
- 5.
A purified antibody that specifically binds an N-methyltransferase present in Limonium latifolium.
A purified antibody that specifically binds a polypeptide consisting of an amino acid sequence selected from the group consisting of:
- SEQ ID NOs:
- 1-5.
A cell into which has been introduced a purified N-methyltransferase, the N-methyltransferase:
- (a) being present in Limonium latifolium;
- (b) having an isoelectric point of about 5.15;
- and
- (c) migrating on SDS-PAGE at about 43 kilodaltons.
A cell into which has been introduced a purified protein, the protein comprising an amino acid sequence selected from the group consisting of:
- SEQ ID NOs:
- 1-5.
Dependencies are inferred from the claim text.