Carbonyl reductase of kluyveromyces marxianus and its isolation and purification method
AssigneeKorea Institute of Science and Technology
InventorsHun-Yeong Koh, Ye-Sun Han, Joo-Hwan Cha, Wook-Hyun Kim, Jong-Soo Lee, Hong-Chul Yun
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Abstract
Carbonyl reductase (CR) of a yeast strain, Kluyveromyces marxianus, and its isolation and purification method thereof is provided. The reduction reaction by the reductase obtained in the present invention is more stereospecific and requires less cost and time than the reduction reactions by the microorganisms or by the reducing enzymes obtained from a chemical synthesis.
Claims4
A carbonyl reductase, separated from Kluyveromyces marxianus and purified, having 40˜42 kDa in size and active at the temperature range of 25˜35° C. and the pH range of 6.5˜7.5.
A method of obtaining carbonyl reductase from Kluyveromyces marxianus including the steps of
- (1) cultivating Kluyveromyces marxianus,
- (2) collecting the cultivated cells from the culture medium by centrifugation and then, lysing the cells, and
- (3) collecting the supernatant after centrifuging the above cell lysate, and separating and purifying the active material against the substrates by subjecting the obtained supernatant to column chromatography.
Dependencies are inferred from the claim text.