Carbohydrate crosslinked glycoprotein crystals

A crosslinked glycoprotein crystal, said crystal being crosslinked through one or more carbohydrate moieties in said glycoprotein and through one or more amino acid side chain functional groups in said glycoprotein.

A method for producing a crosslinked glycoprotein crystal comprising the steps of:

• (a) reacting a glycoprotein crystal with an oxidizing agent to oxidize the carbohydrate moieties of said glycoprotein;
• and
• (b) reacting said glycoprotein crystal with a crosslinking agent, under conditions sufficient to induce crosslinking of said crystal through the carbohydrate moieties of said glycoprotein.

A method for producing a crosslinked glycoprotein crystal comprising the step of reacting a glycoprotein crystal with a first crosslinking agent, said first crosslinking agent being capable of crosslinking said crystal through the amino acid side chain functional groups of said glycoprotein and at least a second crosslinking agent, said second crosslinking agent being capable of crosslinking said glycoprotein through carbohydrate moieties of said glycoprotein.

A method for producing a crosslinked glycoprotein crystal comprising the steps of:

• (a) reacting a glycoprotein crystal with a first crosslinking agent, under conditions sufficient to induce crosslinking through one or more carbohydrate moieties of said glycoprotein;
• and
• (b) reacting said crosslinked glycoprotein crystal with at least a second crosslinking agent, under conditions sufficient to induce crosslinking of said crystal through one or more carbohydrate moieties of said glycoprotein, said second crosslinking agent being different from said first crosslinking agent.

A method for producing a crosslinked glycoprotein crystal comprising the steps of:

• (a) reacting a glycoprotein crystal with a first crosslinking agent, under conditions sufficient to induce crosslinking through one or more amino acid side chain functional groups of said glycoprotein;
• and
• (b) reacting said crosslinked glycoprotein crystal with least a second crosslinking agent, under conditions sufficient to induce crosslinking of said crystal through one or more carbohydrate moieties of said glycoprotein.

A method for producing a crosslinked glycoprotein crystal comprising the steps of:

• (a) reacting a glycoprotein crystal with a first crosslinking agent, under conditions sufficient to induce crosslinking through one or more amino acid side chain functional groups of said glycoprotein;
• and
• (b) reacting said crosslinked glycoprotein crystal with a second crosslinking agent and at least a third crosslinking agent, said second crosslinking agent being capable of crosslinking said glycoprotein through one or more amino acid side chain functional groups of said glycoprotein and said third crosslinking agent being capable of crosslinking said glycoprotein through one or more carbohydrate moieties of said glycoprotein.

A method for producing a crosslinked glycoprotein crystal comprising the steps of:

• (a) reacting a glycoprotein crystal with a first crosslinking agent, under conditions sufficient to induce crosslinking through one or more carbohydrate moieties of said glycoprotein;
• and
• (b) reacting said crosslinked glycoprotein crystal with a second crosslinking agent and at least a third crosslinking agent, said second crosslinking agent being capable of crosslinking said glycoprotein through one or more amino acid side chain functional groups of said glycoprotein and said third crosslinking agent being capable of crosslinking said glycoprotein through one or more carbohydrate moieties of said glycoprotein.