Genomic mammary amyloid a sequence
AssigneeBoard of Regents of the University of Nebraska
InventorsThomas L. McDonald, Marilynn A. Larson, Annika Weber
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Abstract
A genomic nucleotide sequence encoding Serum Amyloid A (SAA), isolated and purified from mammalian colostrum, is disclosed. Methods of use for the same in transgenic protocols is also disclosed.
Claims17
A purified and isolated nucleic acid molecule that encodes colostrum associated SAA wherein said protein encoded by said nucleic acid molecule comprises a TFLK amino acid sequence present in the N-terminal region of said protein said nucleic acid molecule comprising one or more noncoding regions of approximately 50 or more contiguous nucleotides from SEQ ID NO:31 and their conservatively modified variants.
A purified and isolated genomic bovine nucleotide sequence which encodes colostrum associated SAA said sequence comprising a non coding region selected from the group consisting of bases 1-647, 738-2982, 3121-3510, 3674-4984 of SEQ ID NO:31 and those regions that will hybridize under conditions of high stringency to said bases.
A purified and isolated nucleic acid molecule that encodes colostrum associated SAA wherein said protein encoded by said nucleic acid molecule comprises a TFLK amino acid sequence present in the N-terminal region of said protein said nucleic acid molecule comprising SEQ ID NO:31 and its conservatively modified variants.
A method for producing MAA peptide comprising:
- administering a MAA inducing agent to a bovine mammary epithelial cell line;
- culturing said cell so that MAA is produced;
- and
- harvesting said MAA from said mammary epithelial cell.
Dependencies are inferred from the claim text.