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US 2003/0170827 A1Published application
Purified mammalian cytokines; related reagents and methods
AssigneeSchering Corporation, a New Jersey corporation
InventorsDaniel M. Gorman, J. Fernando Bazan, Robert A. Kastelein, Gerard Zurawski
Life of this patent
Estimating the term…
Abstract
CTLA-8 related antigens from mammals, reagents related thereto including purified proteins, specific antibodies, and nucleic acids encoding said antigens. Methods of using said reagents and diagnostic kits are also provided.
Claims20
An isolated or recombinant polynucleotide comprising sequence from:
- a) a mammalian IL-173, which:
- i) encodes at least 8 contiguous amino acids of mature SEQ ID NO:
- 6, 8, 10, or or 12;
- ii) encodes at least two distinct segments of at least 5 contiguous amino acids of mature SEQ ID NO:
- 6, 8, 10, or 12;
- or
- iii) comprises one or more segments at least 21 contiguous nucleotides of SEQ ID NO:
- 5, 7, 9, or 11;
- b) a mammalian IL-174, which:
- i) encodes at least 8 contiguous amino acids of mature SEQ ID NO:
- 14, 16, or 18;
- ii) encodes at least two distinct segments of at least 5 contiguous amino acids of mature SEQ ID NO:
- 14, 16, or 18;
- or
- iii) comprises one or more segments at least 21 contiguous nucleotides of SEQ ID NO:
- 14, 16, or 18;
- c) a mammalian IL-176, which:
- i) encodes at least 8 contiguous amino acids of mature SEQ ID NO:
- 28;
- ii) encodes at least two distinct segments of at least 5 contiguous amino acids of mature SEQ ID NO:
- 28;
- or
- iii) comprises one or more segments at least 21 contiguous nucleotides of SEQ ID NO:
- 27;
- or
- d) a mammalian IL-177, which:
- i) encodes at least 8 contiguous amino acids of mature SEQ ID NO:
- 30;
- ii) encodes at least two distinct segments of at least 5 contiguous amino acids of mature SEQ ID NO:
- 30;
- or
- iii) comprises one or more segments at least 21 contiguous nucleotides of SEQ ID NO:
- 29.
30 more elements
An isolated or recombinant antigenic polypeptide:
- a) (IL-173) comprising at least:
- i) one segment of 8 identical contiguous amino acids from the mature coding portions of SEQ ID NO:
- 6, 8, 10, or 12;
- or
- ii) two distinct segments of at least 5 contiguous amino acids from the mature coding portions of SEQ ID NO:
- 6, 8, 10, or 12;
- b) (IL-174) comprising at least:
- i) one segment of 8 identical contiguous amino acids from the mature coding portions of SEQ ID NO:
- 14, 16, or 18;
- or
- ii) two distinct segments of at least 5 contiguous amino acids from the mature coding portions of SEQ ID NO:
- 14, 16, or 18;
- c) (IL-176) comprising at least:
- i) one segment of 8 identical contiguous amino acids from the mature coding portions of SEQ ID NO:
- 28;
- or
- ii) two distinct segments of at least 5 contiguous amino acids from the mature coding portions of SEQ ID NO:
- 28;
- or
- d) (IL-177) comprising at least:
- i) one segment of 8 identical contiguous amino acids from the mature coding portions of SEQ ID NO:
- 30;
- or
- ii) two distinct segments of at least 5 contiguous amino acids from the mature coding portions of SEQ ID NO:
- 30.
22 more elements
A method of evaluating the selectivity of binding of an antibody to a protein of the mature SEQ ID NO:
- 6, 8, 10, 12, 14, 16, 18, 28, or 30, comprising contacting said antibody to said protein and to another cytokine;
- and comparing binding of said antibody to said protein and said cytokine.
Dependencies are inferred from the claim text.