Fibroblast growth factor-like molecules and uses thereof
OwnerNobuyuki Itohinventor
Life of this patent
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Abstract
Novel FGF-like polypeptides and nucleic acid molecules encoding the same. The invention also provides vectors, host cells, selective binding agents, and methods for producing FGF-like polypeptides. Also provided for are methods for the treatment, diagnosis, amelioration, or prevention of diseases with FGF-like polypeptides.
Claims45
An isolated nucleic acid molecule comprising a nucleotide sequence selected from:
- (a) the nucleotide sequence as set forth in SEQ ID NO:
- 1;
- (b) a nucleotide sequence encoding the polypeptide as set forth in SEQ ID NO:
- 2;
- (c) a nucleotide sequence which hybridizes under moderately or highly stringent conditions to the complement of (a) or (b), wherein the encoded polypeptide has an activity of the polypeptide as set forth in SEQ ID NO:
- 3;
- and
- (d) a nucleotide sequence complementary to any of (a)-(c).
An isolated nucleic acid molecule comprising a nucleotide sequence selected from:
- (a) a nucleotide sequence encoding a polypeptide that is at least about 70, 75, 80, 85, 90, 95, 96, 97, 98, or 99 percent identical to the polypeptide as set forth in SEQ ID NO:
- 3, wherein the polypeptide has an activity of the polypeptide as set forth in SEQ ID NO:
- 3;
- (b) a nucleotide sequence encoding an allelic variant or splice variant of the nucleotide sequence as set forth in SEQ ID NO:
- 1, wherein the encoded polypeptide has an activity of the polypeptide as set forth in SEQ ID NO:
- 3;
- (c) a nucleotide sequence of SEQ ID NO:
- 1;
- (a);
- or (b) encoding a polypeptide fragment of at least about 25 amino acid residues, wherein the polypeptide has an activity of the polypeptide as set forth in SEQ ID NO:
- 3;
- (d) a nucleotide sequence of SEQ ID NO:
- 1, or (a)-(c) comprising a fragment of at least about 16 nucleotides;
- (e) a nucleotide sequence which hybridizes under moderately or highly stringent conditions to the complement of any of (a)-(d), wherein the polypeptide encoded by the nucleotide sequence has an activity of the polypeptide as set forth in SEQ ID NO:
- 3;
- and
- (f) a nucleotide sequence complementary to any of (a)-(c).
An isolated nucleic acid molecule comprising a nucleotide sequence selected from:
- (a) a nucleotide sequence encoding a polypeptide as set forth in SEQ ID NO:
- 3 with at least one conservative amino acid substitution, wherein the polypeptide has an activity of the polypeptide as set forth in SEQ ID NO:
- 3;
- (b) a nucleotide sequence encoding a polypeptide as set forth in SEQ ID NO:
- 3 with at least one amino acid insertion, wherein the polypeptide has an activity of the polypeptide as set forth in SEQ ID NO:
- 3;
- (c) a nucleotide sequence encoding a polypeptide as set forth in SEQ ID NO:
- 3 with at least one amino acid deletion, wherein the polypeptide has an activity of the polypeptide as set forth in SEQ ID NO:
- 3;
- (d) a nucleotide sequence encoding a polypeptide as set forth in SEQ ID NO:
- 3 which has a C- and/or N-terminal truncation, wherein the polypeptide has an activity of the polypeptide as set forth in SEQ ID NO:
- 3;
- (e) a nucleotide sequence encoding a polypeptide as set forth in SEQ ID NO:
- 3 with at least one modification selected from the group consisting of amino acid substitutions, amino acid insertions, amino acid deletions, C-terminal truncation, and N-terminal truncation, wherein the polypeptide has an activity of the polypeptide as set forth in SEQ ID NO:
- 3;
- (f) a nucleotide sequence of (a)-(e) comprising a fragment of at least about 16 nucleotides;
- (g) a nucleotide sequence which hybridizes under moderately or highly stringent conditions to the complement of any of (a)-(f), wherein the polypeptide encoded by the nucleotide sequence has an activity of the polypeptide as set forth in SEQ ID NO:
- 3;
- and
- (h) a nucleotide sequence complementary to any of (a)-(e).
The isolated nucleic acid molecule comprising a nucleotide sequence encoding a polypeptide that is at least about 70, 75, 80, 85, 90, 95, 96, 97, 98, or 99 percent identical to the polypeptide as set forth in SEQ ID NO:
- 3, wherein the polypeptide encoded by the nucleic acid sequence has an activity of the polypeptide as set forth in SEQ ID NO:
- 3, and wherein the percent identity is determined using a computer program selected from the group consisting of GAP, BLASTP, BLASTN, FASTA, BLASTA, BLASTX, BestFit, and the Smith-Waterman algorithm.
An isolated polypeptide comprising the amino acid sequence set forth in SEQ ID NO:
- 3.
An isolated polypeptide comprising the amino acid sequence selected from:
- (a) the mature amino acid sequence as set forth in SEQ ID NO:
- 3;
- (b) the mature amino acid sequence as set forth in SEQ ID NO:
- 3 with an amino-terminal methionine;
- (c) an amino acid sequence for an ortholog of SEQ ID NO:
- 3, wherein the encoded polypeptide has an activity of the polypeptide as set forth in SEQ ID NO:
- 3;
- (d) an amino acid sequence that is at least about 70, 80, 85, 90, 95, 96, 97, 98, or 99 percent identical to the amino acid sequence of SEQ ID NO:
- 3, wherein the polypeptide has an activity of the polypeptide as set forth in SEQ ID NO:
- 3;
- (e) a fragment of the amino acid sequence set forth in SEQ ID NO:
- 3 comprising at least about 25 amino acid residues, wherein the polypeptide has an activity of the polypeptide as set forth in SEQ ID NO:
- 3;
- and
- (f) an amino acid sequence for an allelic variant or splice variant of either the amino acid sequence as set forth in SEQ ID NO:
- 2, or at least one of (a)-(d) wherein the polypeptide has an activity of the polypeptide as set forth in SEQ ID NO:
- 3.
An isolated polypeptide comprising the amino acid sequence selected from:
- (a) the amino acid sequence as set forth in SEQ ID NO:
- 3 with at least one conservative amino acid substitution, wherein the polypeptide has an activity of the polypeptide as set forth in SEQ ID NO:
- 3;
- (b) the amino acid sequence as set forth in SEQ ID NO:
- 3 with at least one amino acid insertion, wherein the polypeptide has an activity of the polypeptide as set forth in SEQ ID NO:
- 3;
- (c) the amino acid sequence as set forth in SEQ ID NO:
- 3 with at least one amino acid deletion, wherein the polypeptide has an activity of the polypeptide as set forth in SEQ ID NO:
- 3;
- (d) the amino acid sequence as set forth in SEQ ID NO:
- 2 or SEQ ID NO:
- 3 which has a C- and/or N-terminal truncation, wherein the polypeptide has an activity of the polypeptide as set forth in SEQ ID NO:
- 3;
- and
- (e) the amino acid sequence as set forth in SEQ ID NO:
- 3, with at least one modification selected from the group consisting of amino acid substitutions, amino acid insertions, amino acid deletions, C-terminal truncation, and N-terminal truncation, wherein the polypeptide has an activity of the polypeptide as set forth in SEQ ID NO:
- 3.
An antibody produced by immunizing an animal with a peptide comprising an amino acid sequence of SEQ ID NO:
- 3.
A hybridoma that produces a monoclonal antibody that binds to a peptide comprising an amino acid sequence of SEQ ID NO:
- 3.
A selective binding agent produced by immunizing an animal with a polypeptide comprising an amino acid sequence selected from the group consisting of SEQ ID NO:
- 2 or SEQ ID NO:
- 3.
Dependencies are inferred from the claim text.