Anti-Fas antibodies
AssigneeSankyo Company, Limited
InventorsNobufusa Serizawa, Kimihisa Ichikawa, Jun Ohsumi, Masahiko Ohtsuki, Hideyuki Haruyama, Tohru Takahashi, Hiroko Yoshida, Akio Shiraishi, Shin Yonehara, Kaori Nakahara, Ikuko Tamaki
Life of this patent
Estimating the term…
Abstract
Anti-Fas antibodies which are cross-reactive with mouse and human Fas and are useful in the treatment of conditions attributable to abnormalities in the Fas/Fas ligand system.
Claims119
A molecule having an antigen binding region specific for an epitope of the Fas antigen, said epitope being conserved between a primate and a non-primate animal.
A molecule having an antigen binding region specific for a conserved, mammalian Fas epitope.
An antibody produced by the hybridoma HFE7A having the accession number FERM BP-5828.
A molecule having at least six antibody CDR's, said antibody being specific for human Fas, wherein said CDR's have identity with the CDR's of the antibody produced by the hybridoma HFE7A having the accession number FERM BP-5828.
A molecule having an antigen binding region, said binding region having specificity for the antigenic determinant recognized by the antibody produced by the hybridoma HFE7A having the accession number FERM BP-5828.
A molecule comprising a light polypeptide chain and a heavy polypeptide chain, the heavy chain having the following formula (I):
- -FRH1-CDRH1-FRH2-CDRH2-FRH3-CDRH3-FRH4- (I)
- wherein FRH1 represents an amino acid sequence having 18 to 30 amino acids, CDRH1 represents the sequence of. SEQ ID NO:
- 2, FRH2 represents an amino acid sequence having 14 amino acids, CDRH2 represents the sequence of SEQ ID NO:
- 3, FRH3 represents an amino acid sequence having 32 amino acids, CDRH3 represents the sequence of SEQ ID NO:
- 4, FRH4 represents an amino acid sequence having 11 amino acids, and each amino acid binds to another amino acid via a peptide bond,
- the light chain having the following formula (II):
- -FRL1-CDRL1-FRL2-CDRL2-FRL3-CDRL3-FRL4- (II)
- wherein FRL1 represents an amino acid sequence having 23 amino acids, CDRL1 represents the sequence of SEQ ID NO:
- 5, FRL2 represents an amino acid sequence having 15 amino acids, CDRL2 represents the sequence of SEQ ID NO:
- 6, FRL3 represents an amino acid sequence having 32 amino acids, CDRL3 represents the sequence of SEQ ID NO:
- 7, FRL4 represents an amino acid sequence having 10 amino acids, and each amino acid binds to another amino acid via a peptide bond.
A humanized molecule having an antigen binding region specific for an epitope of the Fas antigen conserved between a primate and a non-primate animal, said molecule obtained by grafting the respective CDR's from an antibody specific for said epitope of the Fas antigen into each of at least one human light chain, or fragment thereof, and at least one human heavy chain, or fragment thereof.
A DNA comprising a nucleotide sequence selected from the group consisting of the nucleotide sequence 100 to 753 of SEQ ID NO:
- 49, the nucleotide sequence 100 to 753 of SEQ ID NO:
- 51, the nucleotide sequence 100 to 753 of SEQ ID NO:
- 53, the nucleotide sequence 100 to 753 of SEQ ID NO:
- 106 and the nucleotide sequence 100 to 753 of SEQ ID NO:
- 108.
A DNA comprising a nucleotide sequence selected from the group consisting of nucleotide sequence 84 to 2042 of SEQ ID NO:
- 88 and nucleotide sequence 84 to 2024 of SEQ ID NO:
- 116.
A recombinant DNA vector comprising DNA encoding a light chain polypeptide protein selected individually from the group consisting of the amino acid sequence 1 to 218 of SEQ ID NO:
- 50, the amino acid sequence 1 to 218 of SEQ ID NO:
- 52, the amino acid sequence 1 to 218 of SEQ ID NO:
- 54, the amino acid sequence 1 to 218 of SEQ ID NO:
- 107 and the amino acid sequence 1 to 218 of SEQ ID NO:
- 109.
A recombinant DNA vector comprising DNA encoding a heavy chain polypeptide protein selected individually from the group consisting of the amino acid sequence 1 to 451 of SEQ ID NO:
- 89 and the amino acid sequence 1 to 451 of SEQ ID NO:
- 117.
A host cell transformed with a recombinant DNA vector comprising DNA encoding a light chain polypeptide protein selected individually from the group consisting of the amino acid sequence 1 to 218 of SEQ ID NO:
- 50, the amino acid sequence 1 to 218 of SEQ ID NO:
- 52, the amino acid sequence 1 to 218 of SEQ ID NO:
- 54, the amino acid sequence 1 to 218 of SEQ ID NO:
- 107 and the amino acid sequence 1 to 218 of SEQ ID NO:
- 109.
A host cell transformed with a recombinant DNA vector comprising DNA encoding a heavy chain polypeptide protein selected individually from the group consisting of the amino acid sequence 1 to 451 of SEQ ID NO:
- 89 and the amino acid sequence 1 to 451 of SEQ ID NO:
- 117.
A host cell transformed with at least one recombinant DNA vector comprising DNA encoding a light chain polypeptide protein and DNA encoding a heavy chain polypeptide protein,
- said light chain polypeptide protein comprising a sequence selected individually from the group consisting of the amino acid sequence 1 to 218 of SEQ ID NO:
- 50, the amino acid sequence 1 to 218 of SEQ ID NO:
- 52, the amino acid sequence 1 to 218 of SEQ ID NO:
- 54, the amino acid sequence 1 to 218 of SEQ ID NO:
- 107 and the amino acid sequence 1 to 218 of SEQ ID NO:
- 109,
- said heavy chain polypeptide protein comprising a sequence selected individually from the group consisting of the amino acid sequence 1 to 451 of SEQ ID NO:
- 89 and the amino acid sequence 1 to 451 of SEQ ID NO:
- 117.
An E. coli which is selected from the group consisting of E. coli pHSGMM6 SANK73697 (FERM BP-6071), E. coli pHSGHM17 SANK73597 (FERM BP-6072), E. coli pHSGHH7 SANK73497 (FERM BP-6073), E. coli pHSHM2 SANK 70198, E. coli pHSHH5 SANK 70398 (FERM BP-6272), E. coli pgHSL7A62 (FERM BP-6274) SANK 73397 (FERM BP-6074) and E. coli pgHPDHV3 SANK 70298 (FERM BP-6273).
The hybridoma HFE7A having the accession number FERM BP-5828.
A method for producing an antibody having an antigen binding region specific for an epitope of the Fas antigen, said method comprising culturing the hybridoma HFE7A having the accession number FERM BP-5828, and harvesting expressed antibody.
A method for producing an antibody, said method comprising culturing the hybridoma HFE7A having the accession number FERM BP-5828, and harvesting expressed antibody.
A humanized anti-Fas antibody molecule capable of, in cells having an abnormality in the Fas/Fas ligand system,
- (a) inducing apoptosis by binding Fas on the cell surface, and in cells having no such abnormality,
- (b) preventing apoptosis which would otherwise be induced by the binding of Fas ligand to the Fas antigen.
An antibody molecule comprising one or more heavy chain subunits having an amino acid sequence selected from the group consisting of:
- the amino acid sequence 1 to 451 of SEQ ID NO:
- 143;
- the amino acid sequence 1 to 451 of SEQ ID NO:
- 145;
- the amino acid sequence 1 to 451 of SEQ ID NO:
- 147;
- and
- the amino acid sequence 1 to 451 of SEQ ID NO:
- 157.
An antibody molecule, wherein one or more light chain subunits have an amino acid sequence selected from the group consisting of:
- the amino acid sequence 1 to 218 of SEQ ID NO:
- 127;
- the amino acid sequence 1 to 218 of SEQ ID NO:
- 129;
- and
- the amino acid sequence 1 to 218 of SEQ ID NO:
- 131, and one or more heavy chain subunits having an amino acid sequence selected from the group consisting of:
- the amino acid sequence 1 to 451 of SEQ ID NO:
- 143;
- the amino acid sequence 1 to 451 of SEQ ID NO:
- 145;
- and
- the amino acid sequence 1 to 451 of SEQ ID NO:
- 147.
A transformant strain of E. coli selected from the group consisting of FERM BP-6512, FERM BP-6511, FERM BP-6513, FERM BP-6515, FERM BP-6514, FERM BP-6516 and FERM BP-6510.
A first polypeptide protein having an amino acid sequence selected from the group consisting of the amino acid sequence 1 to 218 of SEQ ID NO:
- 127, the amino acid sequence 1 to 218 of SEQ ID NO:
- 129 and the amino acid sequence 1 to 218 of SEQ ID NO:
- 131, together with a second polypeptide protein having an amino acid sequence selected from the group consisting of the amino acid sequence 1 to 451 of SEQ ID NO:
- 143, the amino acid sequence 1 to 451 of SEQ ID NO:
- 145 and the amino acid sequence 1 to 451 of SEQ ID NO:
- 147, said first polypeptide protein and said second polypeptide protein constituting a Fas-specific antibody.
Dependencies are inferred from the claim text.