Method for in vitro propagation of the agent responsible for transmissible spongiform encephalopathies
AssigneeUniversite Joseph Fourier - Grenoble 1
InventorsFrancoise Blanquet Grossard, Jean-Yves Cesbron, Catherine Lemaire Vieille, Jerome Follet
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Abstract
The invention concerns a method for in vitro propagation of the agent responsible for transmissible spongiform encephalopathies, or prion, comprising steps which consist in: providing a culture or a glial cell line and in infecting the culture or line with the agent responsible for transmissible spongiform encephalopathies or prion. Said method enables to obtain infected cell line capable of being used to assess the efficacy of a molecule in the reduction or inhibition of the infectiosity of the prion or for detecting the prion.
Claims17
A method for in vitro propagation of the agent responsible for transmissible spongiform encephalopathies (TSEs), or prion, characterized in that a glial cell culture or a glial cell line is provided and in that said culture or line is infected with the agent responsible for transmissible spongiform encephalopathies (TSEs), or prion.
A line of glial cells infected with the prion, capable of propagating the prion.
A method for detecting and, optionally, quantifying the prion, in a biological sample, characterized in that said sample is brought into contact with a culture or a line of glial cells, under conditions which allow the infection thereof, and in that the infection or noninfection of said line with said sample is detected.
The use of the MSC-80 cell line for propagating the prion in vitro.
Dependencies are inferred from the claim text.