Process for producing glycoprotein
OwnerKenichi Suga, Takeshi Omasa +5inventors
InventorsKenichi Suga, Takeshi Omasa, Michimasa Kishimoto, Yoshio Katakura, Toyoo Ohda, Hideo Miki, Kaoru Kobayashi
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Abstract
A means of controlling sugar chain-modification and sugar chain structure in the production of a glycoprotein with the use of gene recombination techniques. A process for producing a glycoprotein by culturing a gene recombinant host in a medium characterized in that the relative sugar consumption speed is employed as an indication and changed to thereby modify the sugar chain structure attached to the protein.
Claims16
(amended)A method for producing a glycoprotein by culturing a genetically recombinant host in a medium, wherein the sugar chain structure binding to a protein is modified by changing the specific sugar consumption rate (consumption rate of sugar in the medium) as an index, the method comprising the steps of
- (1) measuring the specific sugar consumption rate in the medium, for the setting of a reference value;
- (2) measuring the specific sugar consumption rate after the elapse of a certain culturing period, for the selection of conditions to increase the value thereof relative to the specific consumption rate of the reference value;
- and
- (3) selecting the conditions to increase the value of the specific consumption rate after the elapse of a certain culturing period.
A method for producing a glycoprotein by culturing a genetically recombinant CHO cell in a medium, wherein the ratio of the fucosylated sugar chain in a sugar chain binding to a protein is decreased by increasing of the specific sugar consumption rate as an index.
Dependencies are inferred from the claim text.